基于物种特异性PCR对海鳗鱼糜掺假检测技术的研究

Study on the PCR method specific for muraenesox cinereus surimi adulteration detection technology

为了对海鳗鱼糜掺假进行定性研究,从GenBank数据库下载海鳗及其他物种的线粒体12S rRNA,并通过BioEdit 7.0软件对该基因碱基序列进行比对。在此基础上,综合考虑引物设计原则,设计出关于海鳗的特异性引物。对引物的特异性、灵敏性以及主要加工过程进行实验,结果表明所设计的引物对海鳗DNA具有很强的特异性,灵敏性达到0.1%,加工过程也不影响检测过程,从而满足了对于海鳗鱼糜掺假的检测要求。

In order to carry out qualitative research on muraenesox cinereus surimi adulteration,from the GenBank database download muraenesox cinereus and other species of mitochondrial 12S rRNA,then through the BioEdit 7.0 software base sequence of the gene for comparison. On this basis,consider revising the principle of primer design to design specific primers for muraenesox cinereus. Primer specificity,sensitivity and the main process of the experimental results showed that the designed primers for DNA moray had a strong specificity, sensitivity reached 0.1%,and the process did not affect the detection process,still competent for the muraenesox cinereus identification requirements.