超高效液相色谱法同时测定丹蛭降糖胶囊中丹皮酚和芍药苷含量

Simultaneous determination of the contents of paeonol and paeoniflorin in Danzhi Jiangtang capsule by UPLC

目的:建立一种超高效液相色谱法同时测定丹蛭降糖胶囊中丹皮酚、芍药苷的含量。方法:色谱柱为Waters Acquity UPLC BEH C18(50 mm×2.1 mm,1.7μm)柱,以乙腈-0.1%磷酸为流动相进行梯度洗脱,流速为0.25 mL.min-1,柱温为30℃,检测波长为230 nm。结果:丹皮酚和芍药苷的线性范围分别为0.653～1.959μg(r=0.999 7)和0.108～0.324μg(r=0.999 9),平均加样回收率分别为99.46%,RSD=0.46%(n=6)和99.19%,RSD=0.49%(n=6)。结论:新建方法简便、快速、准确、易行,可用于控制制剂质量。

Objective： To establish a UPLC method for the simultaneous determination of paeonol and paeoniflorin in Danzhi Jiangtang capsule.Methods： Separation was performed on Waters Acquity UPLC BEH C18（50 mm×2.1 mm,1.7 μm） column by a gradient elution using acetonitrile and 0.1% phosphoric acid aqueous solution as the mobile phase at a flow rate of 0.25 mL·min-1 and a column temperature of 30 ℃.The detection wave length was 230 nm.Results： The linearities of paeonol and paeoniflorin were in the range of 0.653~1.959 μg（r=0.999 7） and 0.108~0.324 μg（r=0.999 9）;average recoveries were 99.46%,RSD=0.46%（n=6）,99.19%,RSD=0.49%（n=6）,respectively.Conclusion： The method is accurate,sensitive,reproducible,and suitable for the quality control of Danzhi Jiangtang capsule.