HPLC法同时测定黄芩、白芍药对提取物中8种指标成分的含量

Simultaneous determination of eight ingredients in combination extracts of Scutellaria baicalensis Georgi and Paeonia lactiflora Pall.by HPLC

目的:建立同时测定黄芩、白芍药对提取物中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、芍药苷、芍药内酯苷、没食子酸和12,,3,4,6-五没食子酰葡萄糖8种成分含量的HPLC方法。方法:Agilent ZORBAXSB-C18色谱柱(150 mm×4.6 mm5,μm),流动相组成A:乙腈,B:0.1%磷酸水溶液,梯度洗脱;流速:1.0 mL.min-1,检测波长:230 nm,柱温:30℃。结果:黄芩苷线性范围为186.4～932.0μg,定量下限为186.4μg(r=0.999 8),平均回收率为100.1%(RSD=2.0%,n=6);黄芩素线性范围为19.12～95.60μg,定量下限为19.12μg(r=0.999 7),平均回收率为99.3%(RSD=2.5%,n=6);汉黄芩苷线性范围为44.20～221.0μg,定量下限为44.20μg(r=0.999 5),平均回收率为98.6%(RSD=2.5%,n=6);汉黄芩素线性范围为10.30～51.50μg,定量下限为10.30μg(r=0.999 9),平均回收率为97.5%(RSD=1.4%,n=6);芍药苷线性范围为4.312～43.12μg,定量下限为4.312μg(r=0.999 6),平均回收率为98.4%(RSD=2.4%,n=6);芍药内酯苷线性范围为3.060～30.60μg,定量下限为3.060μg(r=0.999 5),平均回收率为98.2%(RSD=2.0%,n=6);没食子酸线性范围为9.994～199.9μg,定量下限为9.994μg(r=0.999 5),平均回收率为98.8%(RSD=2.1%,n=6);1,2,3,4,6-五没食子葡萄糖线性范围为8.800～44.00μg,定量下限为8.800μg(r=0.999 5),平均回收率为98.0%(RSD=2.2%,n=6)。结论:本方法可应用于黄芩、白芍药对提取物中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、芍药苷、芍药内酯苷、没食子酸和1,2,3,4,6-五没食子酰葡萄糖8种成分含量的同时测定。

Objective：To develop a HPLC method for simultaneous determination of baicalin,baicalein,wogonoside,wogonin,paeoniflorin,albiflorin,gallic acid and 1,2,3,4,6-pentagalloylglucose in combination extracts of Scutellaria baicalensis Georgi and Paeonia lactiflora Pall.Methods：HPLC was performed on an Agilent ZORBAX SB-C18 analytical column（150 mm×4.6 mm,5 μm）with gradient elution of acetonitrile（A）-water containing 0.1% phosphoric acid（B） at the flow rate of 1.0 mL·min-1;detection wavelength was 230 nm,and the column temperature was maintained at 30 ℃.Results：Baicalin,baicalein,wogonoside,wogonin,paeoniflorin,albiflorin,gallic-acid and 1,2,3,4,6-pentagalloylglucose had good linearity in the ranges of 186.4~932.0 μg（r=0.999 8）,19.12~95.60 μg（r=0.999 7）,44.20~221.0 μg（r=0.999 5）,10.30~51.50 μg（r=0.999 9）,4.312~43.12 μg（r=0.999 6）,3.060~30.60 μg（r=0.999 5）,9.994~199.9 μg（r=0.999 5）,and 8.800~44.00 μg（r=0.999 5）,respectively.The average recoveries were 100.1%（RSD=2.0%）,99.3%（RSD=2.5%）,98.6%（RSD=2.5%）,97.5%（RSD=1.4%）,98.4%（RSD=2.4%）,98.2%（RSD=2.0%）,98.8%（RSD=2.1%）,and 98.0%（RSD=2.2%）,respectively.Conclusions：The method can be applied to determine eight ingredients in combination extracts of Scutellaria baicalensis Georgi and Paeonia lactiflora Pall.