酶联免疫吸附试验法检测基础免疫儿童破伤风抗体水平

Detection of Tetanus Antibodies Level in Children with Fundamental Immunity

目的了解0～7岁基础免疫儿童破伤风IgG保护性抗体产生情况。方法采用两种定量酶联免疫吸附试验(ELISA)试剂盒分别检测262份0～7岁基础免疫儿童血清破伤风IgG抗体水平,IgG抗体≥0.1 U/ml为阳性。结果 "安图试剂"IgG抗体阳性146份,阳性率为55.73%;"MP试剂"IgG抗体阳性154份,阳性率为58.78%,两种试剂盒的检测结果间差异无统计学意义(P>0.05)。结论本地区0～7岁基础免疫儿童血清破伤风IgG抗体保护率较低,加强人群破伤风类毒素免疫很有必要。

Objective To understand the producing of tetanus IgG antibodies in children with fundamental immunity aged from 0 to 7 years. Methods Tetanus IgG antibodies of 262 children with fundamental immunity aged from 0 to 7 years were detected by two kinds of ELISA kits. IgG≥0. 1 U/ml was regarded as positive. Results 146 cases were positive by Antu kit and the positive rate was 55.73%, while 154 cases were positive by MP kit and the positive rate was 58.78%. The difference between the two kits was not statistically significant （P 〉 0. 05 ）. Conclusion The level of tetanus IgG antibodies of children with funda- mental immunity is low in local area, and it is necessary for people to enhance their immunity to tetanus toxoid.