鸡致病性大肠杆菌耐药性检测

Detection on drug resistance of chicken pathogenic E.coli

从河南省4个地市(区)商品肉鸡病料中分离了15株大肠杆菌(E.coli),在对其进行生化鉴定的基础上,采用微量倍比稀释法测定了17种抗菌药物对它们的体外最小抑菌浓度(MIC),并按照美国临床实验标准化委员会(NCCLS)的抗菌药物敏感性标准(2009年第十九版)进行耐药性判断和耐药谱比较分析。结果显示:15株鸡E.coli对17种抗菌药物中的16种药物均有不同程度的耐药性,其中对头孢唑林、恩诺沙星、新霉素、环丙沙星的耐药性比例达90%～100%,对阿莫西林、头孢曲松、庆大霉素、阿奇霉素的耐药性比例达到80%以上,对多西环素、大观霉素、左氧氟沙星的耐药性比例达到60%以上。17种抗菌药物敏感性比例达到60%以上的有多粘菌素B、阿米卡星、氟苯尼考,其余药物的敏感性比例均在40%以下。15株鸡E.coli均为多重耐药菌株,耐药谱最多的药物达到14种,耐药谱最少的药物有6种。说明鸡E.coli对常用抗菌药物已产生比较普遍的耐药性,而且耐药谱较宽。

On the basis of their biochemical identification of 15 strains of Escherichia coli （E. coli） isolated from commer- cial chicken in four cities （districts） of Henan Province, the minimum inhibitory concentration （MIC） of 17 kinds of an- timicrobial agents against them in vitro was determined by micro--fold dilution method, and judged their the drug resist- ance and comparatively analyzed the resistance patterns according to the antibacterials susceptibility standard of the Na- tional Committee for Clinical laboratory standardization （NCCLS） （nineteenth edition of 2009）. The results showed 15 strains of chicken E. coli have different levels of resistance to drugs in 16 of the 17 kinds of antimicrobial agents, inclu- ding cefazolin, enrofloxacin, neomycin, ciprofloxacin 90% ~ 100 0~ resistance ratio, amoxicillin, ceftriaxone, gentami- cin, azithromycin resistance ratio more than 80o//00, doxycycline, spectinomycin, levofloxacin resistance ratio more than 60%. 17 kinds of antimicrobial susceptibility ratio more than 60 percent including polymyxin B, amikacin, florfenicol, and the rest of the drug sensitivity ratio below 40~/6. 15 strains of chicken E. coli are multi--resistant strains, the most drug resistance spectrum are 14 kinds, and the least drug resistance spectrum are 6 kinds. It showed that Chicken E. coli has generated widespread resistance to common--used antibiotic drugs and a wide resistance spectrum.