乳香没药对大鼠胆汁分泌的影响

Influence of Olibanum and Myrrha on bile secretion

[目的]观察乳香、没药对大鼠胆汁分泌的影响。[方法]Wistar大鼠连续给予乳香、没药及乳香没药2周,剂量均为生药3 g/kg,对照组给予自来水。末次给药后收集胆汁,分别测试胆汁中总胆汁酸（TBA）、谷胱甘肽（GSH）含量。Western blot法检测肝脏法尼醇X受体（FXR）、胆盐输出泵（BSEP）蛋白表达。各组胆汁及乳香、没药生药用乙酸乙酯萃取,采用高效液相色谱（HPLC）法检测。[结果]乳香、没药及乳香没药组胆汁流量明显高于对照组（P〈0.05）,但各给药组胆汁中TBA、GSH浓度均明显低于对照组（P〈0.05）,通过胆汁分泌的TBA量乳香、乳香没药组显著低于对照组（P〈0.05）,而GSH量各给药组无明显变化。与对照组相比,给药组BSEP蛋白表达量差异无统计学意义,FXR有上调趋势;HPLC图中给药组在2~5 min出现乳香、没药成分色谱峰。[结论]乳香、没药均可使大鼠胆汁分泌量增加,但TBA排出量减少,可能是乳香、没药或其代谢产物在排入胆管时竞争性抑制胆汁酸的排出。

[Objective] To evaluate the influence of Olibanum and Myrrha on bile secretion.[Methods] Wistar rats were treated with Olibanum,Myrrha and Olibanum ＋ Myrrha,3.0 g/kg once a day for 2 weeks and the control group treated with water.After the last administration,bile samples of each group were collected.The level of total bile acid（TBA）,and glutathione（GSH） in bile were determined respectively.Protein levels of farnesoid receptor（FXR）,bile salt export pump（BSEP） were determined by Western blot,bile samples of each group and Olibanum.Myrrha were extracted with ethyl acetate and determined by HPLC.[Results] Compared with control group,three treatment groups had a higher bile flow but lower concentrations of TBA and GSH in bile（P0.05）.The amount of TBA in bile of Olibanum and Olibanum ＋ Myrrha groups increased significantly（P0.05）,while the amount of GSH had no obvious difference to all the treatment groups.Protein level of BSEP had no difference when compared with control group,while FXR had a tendency of increasing.The treatment groups had a peak value of Olibanum and Myrrha at 2~5 min,while control group did not have.[Conclusion] Olibanum and Myrrha can increase the bile secretion favorably,but the amount of TBA in bile is not increased.The probable reason is that Olibanum,Myrrha or their metabolites can competitively inhibit the excretion of TBA when they excreted into bile duct.