细胞间粘附分子-1在心肌缺血再灌注损伤中的表达及其相关性

Expression of Intercellular Adhesion Molecule-1 in Rat Heart with Ischemia/Reperfusion Injury and Its Association with Ischemia/Reperfusion Injury

目的 :观察细胞间粘附分子 1 (ICAM 1 )在心肌缺血再灌注缺损中的表达及其相关性。方法 :42只SD大鼠随机分为 7组 ,建立在体心肌缺血再灌注模型 ,于再灌注前后不同时点取缺血再灌注心肌组织 ,用抗鼠ICAM 1单克隆抗体检测ICAM 1的表达 ,测定丙二醛 (MDA)、一氧化氮(NO)含量。结果 :①再灌注 9h后再灌注区ICAM 1表达上调 ;②心肌MDA含量再灌注 1h时达高峰 ;③心肌NO含量进行性下降。结论 :心肌缺血再灌注区ICAM 1表达上调出现于再灌注晚期 ,可能与延迟性再灌注损伤有关。

Objective:To study the expression of intercellular adhesion molecule 1 in rat heart with ischemia /reperfusion injury and its association with ischemia/reperfusion injury.Methods:Fourty two male Sprague Dawley rats were subjected to coronary artery ligation as ischemia/reperfusion heart model and were randomly divided into seven groups of six rats each.The ischemia time was 30 min.The rat hearts were excised at 0,30,60,360,540,720 min after reperfusion.The ischemic and reperfused myocardium was examined immunohistochemically to localize ICAM 1.Malondialdehyde (MDA) and nitric oxide (NO) contents in the myocardium were also determined by thiobarbituric acid and Griss method,respectively.Results:①At the time of 540 min after reperfusion,expression of ICAM 1 in rat hearts was upragulated.②The MDA values were increased during reperfusion ( P < 0.05 ) and reached the peak 1 hour after reperfusion.③The NO values get lower and lower during reperfusion.Conclusion:Expession of ICAM 1 in rat hearts subjected to ischemia followed by reperfusion was increased in the late stage of reperfusion,which might be associated with delayed reperfusion injury.