慢性阻塞性肺疾病大鼠骨骼肌中NF-κB通路的研究

The NF-κB Pathway Activated in the Skeletal Muscle of COPD Rats

目的:研究COPD大鼠骨骼中磷酸化NF-κB通路蛋白的表达情况及意义。方法:采用气管内滴入脂多糖+反复熏香烟法复制COPD大鼠动物模型。Western blot方法测定大鼠骨骼肌内磷酸化NF-κB蛋白的表达,ELISA法测定大鼠血清及膈肌中IL-1β的表达。结果:Western blot结果显示,COPD大鼠骨骼肌中磷酸化NF-κB蛋白的表达均较对照组明显升高(P<0.01);ELISA结果显示,IL-1β在血清及骨骼肌中的表达均升高(P<0.01);NF-κB与IL-1β水平呈显著正相关(r=0.55,P<0.01)。结论:COPD大鼠骨骼肌中NF-κB表达上调,IL-1β可激活骨骼肌中NF-κB通路。

Objective： To study the role of NF-κB pathway in the skeletal muscle of COPD rats. Methods： The COPD rat model was established by instillation of LPS and exposure to cigarette smoke for 28 days. The protein levels of NF- K B in the skeletal muscle were measured by Western blot. The concentration of IL-1 β in serum and diaphragm were measured by ELISA. Results： Western blot resuit showed that, in the skeletal muscle, compared with the normal control group, the protein expression of NF-K B increased significantly in the model group（P〈0.01）. Compared with the normal control group, in the serum and the diaphragm, the IL-1βwere significantly higher than those of the control group（P〈0.01）. NF- κB was significantly positively correlated with the IL-1β （r=0.55, P〈0.01）. Conclusion： The data shows that the NF-K B pathway is up-regulated in the skeletal muscle of COPD rats, and IL-1β may activate NF- κB pathway in skeletal muscle.