宽果苁菔适应昼夜变化ESTs的分离与分析

Isolation and Analysis of Differential Expressed ESTs from Solms-laubachia eurycarpa(Maxim.) Botsch. between Day and Night

以流石滩地区植物广布种宽果苁菔为实验材料,应用磁珠介导的抑制消减杂交方法,分离昼夜差异基因。随机挑选了136个差异ESTs克隆并进行测序,测序结果使用Blast2go程序进行功能注释和分析。结果表明绝大部分ESTs功能与稳定细胞状态和抗性响应相关,其次与物质能量代谢和信号传导相关,宽果苁菔适应昼夜变化过程的机制非常复杂,本研究为进一步研究高山植物适应流石滩恶劣环境的机制奠定了基础。

The wide-distribution species Solms-laubachia eurycarpa on the scree on Baima snow mountain was used as the experimental material, and the suppression subtractive hybridization （SSH） based on magnetic beads technology was used to isolate the differential genes between day and night. After randomly sequencing 136 differential ESTs, these ESTs were further analyzed by functional annotation by Blast2go. Our results show that most ESTs are related to cellular homeostasis and stress response, followed by material and energy metabolism, and signal transduction. The process of S-L. eurycarpa responsive to daytime environment is very complicated, which lays a good foundation for further studying alpine plants adaptation to harsh environments of scree.