烟草类锌指基因NtZFL基因的功能分析

Functional Analysis of a Zinc-finger-like Gene from Nicotiana tabacum

从烟草cDNA中分离出一个类锌指基因NtZFL,开放读码框321 bp,编码106个氨基。QRT-PCR分析表明MV、H2O2、ABA、冷胁迫处理都提高了NtZFL在烟草的表达,Northern blot分析表明该基因在烟草的不同组织中具有组织特异性,在幼叶和花中表达量较高。亚细胞定位表明NtZFL蛋白是定位在细胞壁中的蛋白。NtZFL基因启动子驱动GUS基因转烟草植株显示在幼苗中整株有表达,但在根部和叶脉处表达量较高。

In the present study, a Zinc-finger-like cDNA （NtZFL） was cloned from Nicotiana tabacum, the gene open reading frame （ORF） was 321 bp in length and encoded a predicated polypeptide of 106 amino acids. Expression of NtZFL in tobacco plant in response to biotic and abiotic stress was studied by QRT-PCR, the results showed that NtZFL was responsive to the treatments of MV, H2O2, ABA and cold. Northern blot analysis showed that NtZFL gene was expressed in different tissues of tobacco plant, achieving maximum in the young leaves and flowers. Subcellular localization analysis showed that the NtZFL protein was localized in the cell wall, GUS activity driven by NtZFL promoter was expressed in the whole of the young plant, but strongly detected in roots and leaf veins.