温郁金二萜类化合物C对脂多糖所致人胃腺癌SGC7901细胞NF-κB活化和炎症因子分泌的影响

Effects of diterpenoid curcumrinol C from ether extract of Radix Curcumae on activity of NF-κB and the expression of inflammatory cytokines in LPS-stimulated human gastric cancer SGC7901 cells

目的:研究温郁金二萜类化合物对脂多糖(LPS)诱导的人胃腺癌SGC7901细胞中炎症因子(TNF-α、IL-1β)分泌影响,并探讨其可能分子机制。方法:以10mg/L的LPS刺激体外培养的SGC7901细胞为炎症模型。酶联免疫法测上清中TNF-α、IL-1β分泌量,蛋白质印迹法检测P65、NF-κB抑制蛋白(IκBα)表达,细胞免疫荧光法检测NF-κB P65蛋白核易位。结果:LPS明显诱导人胃腺癌SGC7901细胞TNF-α、IL-1β分泌,P65蛋白生成增多,并大量易位于细胞核;IκBα蛋白降解增多。温郁金化合物抑制LPS诱导的IκBα蛋白降解及P65核易位。结论:温郁金二萜类化合物可能通过抑制NF-κB活化来实现抗炎作用。

AIM： To observe the effects of diterpenoid curcumrinol C from ether extract of Radix Curcumae on activity of NF-κB and the expression of inflammatory cytokines in LPS- stimulated human gastric cancer SGC7901 cells. METHODS： Cells were normally cultured or incubated with LPS, LPS plus Radix Curcumae. The expression of TNF-α, IL-113 in the culture supernatant was assayed by ELISA. The NF-κB of SGC7901 cell was detected by immunohistochemistry. The protein expressions of P65 and IκB were assayed by Western blot. RESULTS： LPS induced markedly TNF-α, IL-113 and NF-κB P65 release, and expression of P65 in the nuclear were significantly increased, but the expression of IκBa was lower. With Radix Curcumae given, TNF-α, IL-1β and NF-kB P65 decreased and IκB increased. CONCLUSION： Radix Curcumae attenuate the inflammatory cytokine secretion in SGC7901 cells by inhibiting actvation of NF-κB.