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重症急性胰腺炎患者治疗前后血清蛋白组学的变化 被引量:1

Serum proteomic analysis of patient with severe acute pancreatitis before and after treatment
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摘要 目的比较重症急性胰腺炎(SAP)患者治疗前后血清蛋白质组表达差异,寻找能够判断SAP病情好转的血清蛋白质。方法利用表面增强激光解析离子化飞行时间质谱(Sulfate—enhanced laser desorption/ionization time—of-flight mass spectrometry,SELDI—TOF-MS)分析20例SAP患者治疗前及治疗后7d的血清蛋白质谱,并进行APACHEII评分。结果SAP患者治疗后7d的APACHEII评分为(10.70±3.47)分,显著低于入院时的(13.00±2.21)分(t=4.272,P=0.002)。治疗前后一共检测到血清中132个蛋白质峰,其中质荷比2000至40000间有27个差异蛋白质峰(P〈0.05)。分子质量47000的蛋白在SAP病情缓解后表达下调,分子质量115000及159000的蛋白在SAP治疗后明显上调。结论用SELDI—TOF—MS技术能检出SAP患者治疗前后血清蛋白质的差异表达,分子质量115000和159000蛋白的上调及47000蛋白的下调可能是SAP病情好转的征兆。 Objective To evaluate the different expression of the serum proteome of patient with severe acute pancreatitis (SAP) before and after treatment, and search for the protein that can predict SAP improvement. Methods SELDI-TOF-MS was used to analyze the expression of serum protein spectrum of 20 SAP patients before treatment and 7 days after treatment, and make the APACHE Ⅱ score at the same time. Results The APACHE Ⅱ score was 10.70 ± 3.47 in patients with SAP 7 days after treatment, which were significantly lower than that before treatment ( 13.00 ± 2.21, P 〈0.05 ). One hundred thirty-two spectral peak clusters were detected before and after treatment, there were 27 proteins that had significantly different signal intensities had been detected from 2000 Da to 50000 Da (P 〈 0.05 ). The peak cluster at 47 000 Da was significantly down-regulated after SAP attenuation. While the peak clusters at 11 700 Da and 15 900 Da were significantly up-regulated after treatment. Conclusions Serum analysis with SELDI-TOF MS can detect the different expression of the SAP serum proteomics before and after treatment, the increased expression of the protein with molecular weight of 11 500 Da and 15 900 Da and the decreased expression of 4700 Da may be a signal of improvement of SAP.
出处 《中华胰腺病杂志》 CAS 2012年第3期147-149,共3页 Chinese Journal of Pancreatology
基金 湖北省自然科学基金(131349)
关键词 胰腺炎 急性坏死性 蛋白质组 血清 预测和判断 Pancreatitis, acute necrotizing Proteome Serum Forecasting
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参考文献9

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