热休克蛋白B1在胰腺癌组织中的表达及临床意义

Detection of HSPB1 expression in pancreatic cancer and its clinical significance

目的检测热休克蛋白B1（HSPB1）在胰腺癌组织中的表达，分析其与肿瘤临床病理特征间的关系。方法选取47例胰腺癌、13例癌旁组织、3例慢性胰腺炎组织标本，用组织芯片构建仪制备组织芯片，应用免疫组织化学sP法检测HSPB1的表达。以阳性细胞所占的比例和染色强度两者评分乘积为标准进行结果判断。结果胰腺癌、癌旁和慢性胰腺炎组织中HSPB1的阳性表达率分别为80．9％（38／47）、12．5％（2／16），两者差异具有统计学意义（X。=24．058，P=0．000）。胰腺癌组织HSPB1的表达与患者性别、年龄及肿瘤部位、组织学类型、神经浸润均无关（P值均〉0．05）。结论HSPB1参与了胰腺癌的发生、发展，这一过程可能是早期的分子事件。

Objective To investigate the expression of HSPB1 in pancreatic cancer and its relationship with clinicopathological characterization. Methods Pathological specimens from 47 cases of pancreatic cancers, 13 cases of para-cancerous normal pancreatic tissues and 3 cases of chronic pancreatitis tissues were selected, and tissue microarray construction instrument was used to prepare tissue microarray, then the expression of HSPB1 was determined by immunohistochemistry SP method. The scores of the proportion of positive cells and staining intensity were multiplied to make the judgment. Results The expression of HSPB1 in malignant, para-cancerous and chronic pancreatitis tissues was 80.9% （ 38/47 ）, 12.5% （ 2/16 ）, respectively; and the difference between the two groups was statistically significant （X2 =24.058, P =0.000）. The expression of HSPB1 in pancreatic cancer tissue was not significantly related to sex, age, location, differentiation degree and nerve infiltration of tumor （P 〉 0.05 ）. Conclusions The expression of HSPB1 is related to development and progression of pancreatic cancer, and may be an early molecular event.