温和高温下胰腺癌细胞株PANC1对吉西他滨耐药性的影响

Mild hyperthermia affects chemoresistance of gemcitabine in pancreatic cancer PANC1 cell line

目的观察温和高温下胰腺癌细胞株PANC1对吉西他滨敏感性的影响。方法应用1μmol／L吉西他滨处理胰腺癌PANC1细胞1h后，分别置37、42、45℃水浴锅孵育1h，再继续培养0、24、48h。采用CCK．8法检测细胞增殖，AV／PI染色后上流式细胞仪检测细胞凋亡率。结果42℃及45℃孵育细胞24、48h后的细胞增殖均较37℃孵育的细胞显著降低（0．96±0．05、0．88±0．03比1．05±0．02：1．28±0．04、0．94±0．04比1．49±0．09；t值分别为4．367、25．120、3．510、12．101，P值均〈0．05），且45℃孵育的细胞增殖显著低于42℃孵育的细胞（￡值分别为3．348、11．732，P值均〈0．05）。37、42、45℃孵育的吉西他滨处理的PANC1细胞48h后的细胞凋亡率分别为（7．125±0．064）％、（9．985±0．615）％、（14．845±1．987）％，3组间的差异均具有统计学意义（t值分别为10．320、9．832、4．575，P值均〈0．05）。结论温和高温可以增加PANC1细胞对吉西他滨的敏感性。

Objective To investigate the effect of mild hyperthermia on chemoresistance of gemcitabine in the pancreatic cancer cell line PANC1. Methods The PANC1 cell was treated with gemcitabine （1 μmol/L） for 1 h, then was heated at 37℃, 42℃ and 45℃ for 1 hour, and was cultured for 0 h, 24 h, 48 h. Cell growth was analyzed by CCK-8 assay. Cell apoptosis was analyzed by Annexin V- fluorescein isothiocyanate （ FITC）/propidium iodide （PI） staining. Results The proliferation of cells at 42℃ and 45℃ for 24 h and 48 h were significantly lower than that at 37℃ （0.96 ± 0.05,0.88 ± 0.03vs 1.05 ± 0.02;1.28 ±0.04,0.94 ±O. 04vs 1.49 ±0.09;t =4.367, 25. 120, P 〈0.05）. The proliferation of cells at 45℃ was significantly lower than that at 42℃ （ t = 3. 348, 11. 732, P 〈 0.05 ）. The cell apoptosis rates at 37℃, 42℃, 45℃ after 48 h were （7. 125 ± 0. 064） %, （9. 985 ± 0. 615 ） %, （ 14. 845 ± 1. 987 ） %, the difference among the 3 groups was statistically significant （ t = 10.320, 9. 832, 4.575, P 〈0.05 ）. Conclusions Mild hyperthermia can reduce chemoresistance of gencitabine in pancreatic cancer cell PANC1.