姜黄素干预对乙二醇诱导的大鼠肾草酸钙结石形成的影响

Effects of curcumin intervention on ethylene glycol-induced calcium oxalate nephrolithiasis in rats

目的研究姜黄素对乙二醇诱导的大鼠肾草酸钙结石形成的影响。方法 32只Wistar大鼠分为单纯诱石组(1%乙二醇自由饮用诱导肾草酸钙结石形成)、诱石+姜黄素干预组(1%乙二醇自由饮用+20 mg.kg-1.d-1姜黄素灌胃)、单纯姜黄素组(去离子水自由饮用+20 mg.kg-1.d-1姜黄素灌胃)和空白对照组(去离子水自由饮用),每组8只。于实验前和实验结束时(分组处理后4周末),分别检测大鼠血、尿离子钙和镁浓度、尿草酸、尿枸橼酸及血清肌酐浓度;处死大鼠后取肾脏组织,检测肾脏组织丙二醛(MDA)含量及总超氧化物歧化酶(T-SOD)活性;HE染色观察肾脏组织草酸钙结晶形成情况;TUNEL法检测肾小管上皮细胞凋亡。结果与空白对照组比较,单纯姜黄素组大鼠尿草酸、肾小管上皮细胞凋亡指数、肾脏组织MDA含量和血清肌酐水平轻度升高,尿镁离子浓度、尿枸橼酸盐含量和肾脏组织T-SOD活性轻度降低。与单纯诱石组比较,诱石+姜黄素干预组大鼠尿草酸、肾小管上皮细胞凋亡指数、肾脏组织MDA含量明显下降,尿镁离子浓度、尿枸橼酸盐含量、肾脏组织T-SOD活性显著升高,组间比较差异均有统计学意义(P<0.05)。结论姜黄素对正常饮食大鼠具有轻微的诱发肾草酸钙结石的作用,但可明显抑制乙二醇诱导的大鼠肾结石生成。

Objective To investigate the effects of curcumin intervention on ethylene glycol-induced calcium oxalate nephrolithiasis in rats.Methods Thirty-two Wistar rats were randomly divided into single nephrolithiasis induction group（calcium oxalate nephrolithiasis induced by 1% ethylene glycol drinking）,nephrolithiasis induction＋curcumin intervention group（1% ethylene glycol drinking＋intragastric administration of 20 mg·kg-1·d-1 curcumin）,single curcumin group（deionized water drinking＋ intragastric administration of 20 mg·kg-1·d-1 curcumin） and blank control group（deionized water drinking）,with 8 rats in each group.Before and after the experiment（4 weeks after treatment）,serum and urine concentrations of ionized calcium and magnesium,urine oxalic acid,urine citric acid and serum concentration of creatinine were measured in rats.Rats were sacrificed,renal tissues were obtained,the content of malondialdehyde（MDA） and activity of total superoxide dismutase（T-SOD） in renal tissues were determined,the formation of calcium oxalate crystals in renal tissues was observed with HE staining,and the apoptosis of renal tubular epithelial cells was detected by TUNEL method.Results Compared with blank control group,the urine oxalic acid,apoptosis index of renal tubular epithelial cells,content of MDA in renal tissues and serum concentration of creatinine were moderately higher,and the urine concentration of ionized magnesium,urine content of citric acid and activity of T-SOD in renal tissues were moderately lower in single curcumin group.Compared with single nephrolithiasis induction group,the urine oxalic acid,apoptosis index of renal tubular epithelial cells and content of MDA in renal tissues were significantly decreased,and the urine concentration of ionized magnesium,urine content of citric acid and activity of T-SOD in renal tissues were significantly increased in nephrolithiasis induction＋curcumin intervention group（P〈0.05）.Conclusion Curcumin can slightly induce calcium oxalate nephrolithiasis in rats with normal diet,but it can effectively inhibit ethylene glycol-induced calcium oxalate nephrolithiasis.