富氢水对高草酸尿所致大鼠肾小管上皮损伤的保护作用研究

Protective effect of hydrogen-rich water on injury of renal tubular epithelial cells induced by hyperoxaluria in rats

目的观察高草酸尿对大鼠肾小管上皮细胞凋亡的影响及富氢水对该影响的作用。方法 56只雄性Wistar大鼠随机分为空白对照组(饮用去离子水,去离子水灌喂)、单纯诱石组(饮用1%乙二醇溶液,去离子水灌喂)、单纯富氢水对照组(饮用去离子水,氧化还原电位-140 mV的高浓度富氢水灌喂)、氢氧化镁对照组(饮用1%乙二醇溶液,氢氧化镁溶液灌喂)以及高、中、低浓度富氢水干预组(饮用1%乙二醇溶液,氧化还原电位-140 mV、-110 mV或-60 mV的富氢水灌喂),每组8只,连续处理4周。测定各组大鼠24 h尿草酸浓度,TUNEL法检测肾小管上皮细胞凋亡指数(AI),测定肾组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果单纯诱石组、氢氧化镁对照组和高、中、低浓度富氢水干预组24 h尿草酸浓度均显著高于空白对照组和单纯富氢水对照组(P<0.05)。单纯诱石组肾小管上皮细胞AI显著高于空白对照组(P<0.05),高、中、低浓度富氢水干预组肾小管上皮细胞AI分别为单纯诱石组的46.8%、60.0%、92.6%,呈显著量效关系(r=-0.724,P<0.05)。富氢水浓度与肾组织SOD活性呈显著正相关(r=0.683,P<0.05),与肾组织MDA含量呈显著负相关(r=-0.736,P<0.05)。结论高草酸尿可造成肾小管上皮细胞凋亡增加,富氢水对此有显著的保护性作用。

Objective To investigate the effect of hyperoxaluria on apoptosis of rat renal tubular epithelial cells,and observe the interventional role of hydrogen-rich water.Methods Fifty-six male Wistar rats were randomly divided into blank control group（drinking deionized water plus treating with deionized water）,calculi-induced group（drinking 1% ethylene glycol plus treating with deionized water）,hydrogen-rich water control group（drinking deionized water plus treating with hydrogen-rich water of-140 mV oxidation-reduction potential）,magnesium hydroxide control group（drinking 1% ethylene glycol plus treating with magnesium hydroxide）,and high,medium and low concentration hydrogen-rich water intervention groups（drinking 1% ethylene glycol plus treating with hydrogen-rich water of-140 mV,-110 mV and-60 mV oxidation-reduction potentials respectively）,with 8 rats in each group.Four weeks after management,24 h oxaluria concentrations were measured,the apoptosis indexes（AI） of renal tubular epithelial cells were detected with TUNEL method,and the contents of malondialdehyde（MDA） and activity of superoxide dismutase（SOD） in renal tissues were determined.Results Twenty-four hour oxaluria concentrations in calculi-induced group,magnesium hydroxide control group and high,medium and low concentration hydrogen-rich water intervention groups were significantly higher than those in blank control group and hydrogen-rich water control group（P〈0.05）.AI of renal tubular epithelial cells in calculi-induced group was significantly higher than that in blank control group（P〈0.05）.AI of renal tubular epithelial cells in high,medium and low concentration hydrogen-rich water intervention groups were 46.8%,60.0% and 92.6% respectively of that in calculi-induced group,which exhibited a significant dose-effect relationship（r=-0.724,P〈0.05）.The concentration of hydrogen-rich water was significantly positively related to the activity of SOD（r=0.683,P〈0.05）,and was significantly negatively related to the content of MDA（r=-0.736,P〈0.05）.Conclusion Hyperoxaluria can increase the apoptosis of renal tubular epithelial cells,and hydrogen-rich water can effectively protect renal tubular epithelial cells from hyperoxaluria-induced injury.