三七抗肝纤维化的实验研究

Effects of Panax Notogenseng on CCl4-induced liver fibrosis in rats

目的探讨三七对四氯化碳(CCl4)中毒性肝纤维化的防治作用。方法复制大鼠CCl4 中毒性肝纤维化模型 ,并以秋水仙碱作为阳性治疗对照。采用光镜、电镜观察组织学改变 ,测定血清丙氨酸氨基转移酶(ALT)、白蛋白、球蛋白、透明质酸(HA)、层粘连蛋白(LN)、肝组织羟脯氨酸(Hyp)含量 ,以反映肝细胞损伤及肝纤维化程度。将形态改变与功能变化相结合 ,对比分析三七对CCl4 中毒性肝纤维化的防治作用。结果三七治疗后肝细胞损害、肝脏脂肪变性、炎细胞浸润的程度均较轻 ,成纤维细胞和胶原纤维增生亦较少。结论三七对肝细胞损伤有一定保护作用 ,并可明显抑制肝组织中成纤维细胞及胶原纤维增生 ,是较理想的防治肝纤维化的药物。

Objective To investigate the effects of Panax Notoginseng (PN) on liver fibrosis (LF) induced with CCl4. Methods The model of CCl4-induced LF was established in rats and they were treated with PN or colchicine (Col). The histological changes were obseved with light and electron microscopy. Serum level of alanine aminotransferase (ALT), albumin, globulin, hyaluronic acid (HA) and laminin (LN) and hydroxyproline (Hyp) in liver tissues were determined to reveal the extent of liver damages. The Col-treated group served as the positive control. Results The extent of liver damages, fatty degeration and infiltration of inflammatory cells were less severe in the PN treated group than in the Col-treated group. The formation of fibroblasts and collagenous fibers was less in amount in the PN-treated group than in the Col-treated group.Conclusion PN exerts a protective effect on CCl4-induced LF in rats and inhibits the formation of fibroblasts and collagenous fibers in liver tissues.