人参皂甙对创伤失血性休克免疫功能的调节作用

Effects of ginsenoside on immunomodulation after traumatohemorrhagic shock in rats

目的探讨人参皂甙(GS)对创伤失血性休克免疫功能的调节作用。方法分体内实验和体外实验两个部分。体内实验 :给大鼠GS预处理3d后进行创伤失血性休克模型的复制 ,1d后处死动物 ,分别测定淋巴细胞和巨噬细胞的功能 ;体外实验 :创伤失血性休克后1d处死动物 ,分离、纯化淋巴细胞和腹腔巨噬细胞 ,观察GS对免疫功能的影响。结果和正常对照组大鼠相比 ,创伤失血性休克1d大鼠脾淋巴细胞增殖、IL 2活性、IL 2R表达均降低 ;巨噬细胞MHC Ⅱ抗原表达、TNF α释放降低 ,巨噬细胞的吞噬能力减弱。GS体内应用(2.5mg/100g)对创伤失血性休克大鼠脾淋巴细胞和巨噬细胞的免疫功能有明显的改善作用 ,表现在脾淋巴细胞增殖能力增强 ,脾细胞IL 2产生、IL 2R表达增加 ,巨噬细胞MHC Ⅱ抗原表达、TNF α释放及巨噬细胞的吞噬能力明显提高 ;一定浓度范围内的GS(1～100μg/ml)在体外应用对创伤失血性休克大鼠1d脾淋巴细胞和巨噬细胞的功能也有不同程度的改善作用。结论人参皂甙在体内和体外对创伤失血性休克大鼠免疫功能抑制均有一定的改善作用。

Objective To study the modulation effects of ginsenoside (GS) on the immune function in traumatohemorrhagic shock in rats. Methods The study was divided into in vivo and in vitro part. In the in vivo sutdy, the rats were pretreated with gensinoside 2.5 mg/100 g for 3 days and inflicted with traumatohemorrhagic shock. The rats were killed on the next day and the functions of their lymphocytes and macrophages were observed. In the in vitro study, the rats were inflicted with traumatohemorrhagic shock and killed on the next day. The lymphocytes in the spleen and the macrophages in the peritoneal cavity were isolated and purified. Their immune functions after the treatment of 1 to 100 μg/ml of GS were studied. Results In the in vivo study, the pretreatment of GS resulted in enhanced proliferative capacity, elevated IL-2 releasing and increased IL-2R expression in splenocytes and enhanced antigen presentation, increased TNF releasing and phagocytosis in peritoneal macrophages. In the in vitro study, administration of GS also improved the functions of splenocytes and peritoneal macrophages. Conclusion Our findings suggest that GS administration in vivo and in vitro is able to improve the immune functions of lymphocytes and macrophages in traumatohemorrhagic shock.