响应面分析南方红豆杉黄酮微波提取工艺研究

Study on Microwave Extraction of Total Flavones from the Leaves and Branches of Taxus mairei(Lemee et Levl.) S. Y. Hu ex Liu by Response Surface Method

目的:优化南方红豆杉[Taxus mairei(Lemee et Levl.)S.Y.Hu ex Liu]枝叶中总黄酮的微波提取工艺。方法:在单因素实验基础上,采用响应面分析法对影响南方红豆杉枝叶中总黄酮提取率的主要因素(提取时间、料液比和乙醇体积分数)进行优化,建立影响因素与响应值之间的数学模型,确定最佳微波提取工艺条件。结果:南方红豆杉枝叶中总黄酮最佳微波提取工艺为:料液比27.39(V/W),乙醇体积分数61.44%,提取时间15.27min,微波频率1 887MHz。结论:采用响应面法对南方红豆杉中总黄酮的提取条件进行优化,该方法合理可靠,为生产工艺提供理论依据。

Objective： The research aimed to optimize the extraction technology of total flavones from the leaves and branches of Taxus mairei （ Lemee et Levi. ） S. Y. Hu ex Liu. Method ： Based on the single factor experiment, the main elements （the extraction time, the microwave frequency, the ratio between liquid and solid and the ethanol concentration） affecting the extraction rate of total flavones from the leaves and branches of Taxus mairei （ Lemee et Levi. ） S. Y. Hu ex Liu were optimized by using response surface analysis, and the functional relationship between the response value and elements was established. Result： The result of the experiment showed the optimum extraction condition as follow： 27.39 times of water, 61.44% ethanol, extracting 15.27 mins by 1 887 MHz microwave frequency. Conclusion： It is feasible and reliable to optimize the conditions of extracting the total flavones from the leaves and branches of Taxus mairei （Lemee et Levi. ） S. Y. Hu ex Liu by response surface analysis, which lays the theoretical foundation to its production process.