视网膜下腔相关的免疫偏离

Subretinal space-associated immune deviation

目的 确立视网膜下腔是否具有支持针对视网膜可溶性抗原 (S抗原 )刺激诱导偏离式免疫反应的能力。方法 将视网膜 S抗原接种于 Wistar大鼠的眼前房和视网膜下腔。抗原接种后 7d,使用 S抗原和完全福氏佐剂免疫受主动物。然后 ,通过足部刺激评估迟发型超敏反应 (DTH)。结果 前房和视网膜下腔注射 S抗原的动物没有发生抗原特异性 DTH。与此相反 ,而未预先进行前房和视网膜下腔抗原接种组动物和结膜下 S抗原接种组动物则诱发了剧烈的 DTH。结论 视网膜下腔针对可溶性抗原具有诱导免疫偏离的能力。

Objective To determine whether the subretinal space supports the induction of deviant immune responses to retinal soluble(S) antigen.Methods Retinal S antigen was inoculated into the anterior chamber(AC) and subretinal space(SR) in Wistar rats.Seven days after antigen inoculation,the recipient animals were immunized with S antigen and complete Freund's adjuvant.Delayed type hypersensitivity(DTH) was assessed by footpad challenge.Results Antigen specific DTH did not develop in rats in which S antigen was injected into the AC and the SR.By contrast,control animals and recipients of subconjunctival injection displayed intensive DTH.Conclusion The subretinal space supports immune deviation for soluble antigen by activity suppressing antigen specific DTH.