喉鳞癌组织中表皮生长因子受体、环氧合酶-2的表达及其临床意义

The expression and clinical significance of epidermal growth factor receptor and cyclooxygenase-2 in laryngeal squamous cell carcinoma

目的检测喉鳞癌组织中表皮生长因子受体（EGFR）和环氧合酶-2（COX-2）的表达，探讨其相关的临床意义。方法收集54例喉鳞癌手术切除组织（喉鳞癌组）及癌旁非癌喉黏膜组织（癌旁对照组）。按组织分化程度、颈淋巴结有无转移、临床TNM分期进行分组比较EGFR和COX-2的表达。结果EGFR在喉鳞癌组的阳性表达率为63．0％（34／54），高于癌旁对照组的42．6％（23，54）（P〈0．05），COX-2在喉鳞癌组的阳性表达率为68．5％（37/54），高于癌旁对照组的35．2％（19／54）（P〈0．01）。喉鳞癌组中EGFR和COX-2的表达量分别为0．584±0．136和0．561±0．134，而癌旁对照组分别为0．161±0．045和0．114±0．027，两组比较差异有统计学意义（P〈0．01）。EGFR和COX-2的表达量在不同组织分化程度、颈淋巴结有无转移、不同临床TNM分期之间比较差异有统计学意义（P〈0．05或〈0．01）。相关性分析显示喉鳞癌组织中EGFR与COX-2的表达呈正相关（r=0．602，P〈0．01）。结论EGFR和COX-2在喉鳞癌的发生发展中起一定的作用，在肿瘤良恶性的鉴别诊断、临床分期以及判断预后等方面，具有显著的临床意义。

Objective To detect the expression of epidermal growth factor receptor （EGFR） and cyclooxygenase-2（COX-2） in laryngeal squamous cell carcinoma and to investigate their clinical significance. Methods Resected tissue of laryngeal squamous cell carcinoma （observation group）and adjacent normal laryngeal mueosa （control group） from 54 patients were collected. According to histological differentiation degree, cervical lymph node metastasis, clinical TNM staging, EGFR and COX-2 were compared. Results The expression rate of EGFR in observation group [63：0%（34/54） ] was higher than that in control group [ 42.6% （23/54）] （P 〈 0.05 ）. And COX-2 in observation group [ 68.5% （37/54）] was higher than that in control group [35.2% （19/54）] （P 〈 0.01 ）. The expression of EGFR and COX-2 in observation group （0.584 ±0.136,0.561 ±0.134） were significantly higher than those in control group （0.161±0.045, 0.114 ± 0.027）, respectively （P 〈 0.01）. The expression of EGFR and COX-2 had significant difference among histological differentiation degree, with or without cervical lymph node metastasis, different clinical TNM staging （P 〈 0.05 or 〈 0.01 ）. Correlation analysis showed that the expression of EGFR and COX-2 in laryngeal squamous cell carcinoma had positive correlation （r = 0.602,P 〈0.01）. Conclusions The expression of EGFR and COX-2 have some contribution to the incidence and development of laryngeal squamous cell carcinoma. They can be used for the different diagnosis of benign and malignant carcinoma,clinical staging and prognosis estimating. They have significant clinical values.