高压氧预处理对减压病大鼠肺组织的影响

Hyperbaric Oxygen Pretreatment on Rat Lung Tissue in Decompression Sickness

目的:研究高压氧预处理对减压病大鼠肺组织的影响及其意义。方法:SD大鼠30只,随机分为正常对照(CN)组,高压氧预处理(HBO)组,减压(DCS)组,减压组采用20min匀速升压至7.0ATA,停留20min使大鼠充分换气,2min内快速减压常压方案。减压24h后观察肺组织中谷胱甘肽过氧化物酶(GPx)、丙二醛(MDA)、超氧化物歧化酶(SOD)的变化;并通过HE染色观察肺组织病理学变化。结果:减压组肺泡腔不够完整,肺泡破裂融合,肺泡壁增厚,有中度炎性细胞浸润,高压氧组与减压组相比,病理改变明显减轻;与对照组相比,单纯减压使大鼠肺组织GPx、MDA升高,SOD降低,高压氧预处理组GPx、MDA降低,SOD降低升高;高压氧组与减压组相比GPx、MDA下降,SOD升高(P<0.05)。结论:高压氧预处理对减压病大鼠肺组织具有一定的保护作用。

Objective： To investigate the effect of hyperbaric oxygen pretreatment on decompression sickness in rat lungs.Methods： SD rats were randomly divided into normal control（CN） group,hyperbaric oxygen preconditioning（HBO） group,decompression（DCS） group,decompression group by using 20min uniform boost to 7.0ATA,stay 20min in rats full ventilation,rapid decompression to atmo-spheric pressure program within 2min.Lung tissue glutathione peroxidase（GPx）,malondialdehyde（MDA）,superoxide dismutase（SOD） changes were detected after decompression 24h;Pulmonary histopathological changes was detecte by HE staining.Results： The alveolar cavity of the decompression is incomplete alveoli rupture integration,alveolar wall thickening,moderate inflammatory cell infiltration,the hyperbaric oxygen group significantly reduced pathological changes compared with that in the decompression group;Compared with that in the control group,simple decompression GPx,rat lung tissue of MDA increased,SOD decreased,GPx,of hyperbaric oxygen pre-treatment group of MDA to reduce of SOD to reduce elevated;Hyperbaric oxygen group and the decompression group compared with GPx,MDA decreased,SOD increased（P0.05）.Conclusion： Hyperbaric oxygen preconditioning has a protective effect of decompression sickness in rat lungs.