单人建立大鼠原位肝移植模型的手术体会

Technique of Orthotopic Liver Transplantation in Rats Operated by One Person without Surgical Microscope

目的:探讨单人建立稳定大鼠原位肝移植模型手术中的难点及对策。方法:单人及双人裸视下采用改良二袖套法制备大鼠原位肝移植模型各50例。结果:单人及双人组供体手术时间、无肝期、受体手术手术时间分别为:(39.16±2.89)min和(38.36±3.04)min、(19.92±1.36)min和(19.70±1.40)min、(61.98±3.46)min和(58.65±3.94)min;单人及双人组手术成功率、1周存活率、一月存活率分别为:94.0%(47/50)和92.0%(46/50)、86.0%(43/50)和88.0%(44/50)、86%(43/50)和84%(42/50)。结论:通过改进手术方法,简化操作,单人即可建立稳定的大鼠原位肝移植模型。

Objective： To investigate the difficulties and countermeasures of the surgical technique in establishing a stable rat model of orthotopic liver transplantation.Methods： The model was established with improved two cuff technique.One hundred cases of rat orthotopic liver transplantation were performed by single person and double persons without surgical microscope.Fifty cases were operated in each group.Results： In single operator group and double operators group,donor operation time,anhepatic time and receptor operation time were（39.16±2.89）min vs（38.36±3.04） min,（19.92±1.36） min vs（19.70±1.40） min,（61.98±3.46） min vs（58.65±3.94） min;The successful operation rate,one week survival rate and one month survival rates in single operator group and double operators group were 94.0 %（47/50） vs 92.0 %（46/50）,86.0 %（43/50） vs 88.0%（44/50）,86 %（43/50） vs 84 %（42/50） respectively.Conclusion： Through improving technique,it is successful to establish a stable model of rat orthotopic liver transplantation by single operator without surgical microscope.