摘要
目的:研究弥漫大B淋巴瘤(Diffuse Large B-Cell Lymphoma,DLBCL)12号染色体基因表达情况。方法:收取临床DLBCL病人淋巴结标本液氮速冻,快速冷冻切片,采用激光显微切割技术分离单纯淋巴瘤细胞,提取淋巴瘤细胞中的mRNA与表达谱芯片杂交,通过信号扫描、处理后获得表达基因杂交信号强度。每基因设11-20对探针。杂交信号与错配探针对比,扣除背景值后,使用Wilcoxon符号秩和检验选取与错配杂交信号有显著差异的基因作为分析结果(P=0.05)。随机选取两个检测到的基因,使用PCR方法检验基因芯片结果的可靠性。结果:成功地从快速冷冻保存的DLBCL标本中提取了RNA。使用表达谱芯片进行研究,发现了共164条12号染色体编码的基因在淋巴瘤细胞中表达。并根据胞内定位,基因功能和基因所属的代谢通路三种分类方法对所得基因进行分类分析。基因表达密度分析显示12号染色体上的基因表达情况与编码基因分布情况比较一致。结论:使用表达谱芯片研究了12号染色体上的基因表达情况,为研究DLBCL提供了依据。
Objective： To explore the profile of expressed genes encoded by chromosome 12 in diffuse large B-cell lymphoma（DLBCL）.Methods： DLBCL lymph node and reactive lymph node were flash frozen in liquid nitrogen.Lymphoma cells were separated from frozen section by Laser Capture Microdissection（LCM）.The mRNA of the cells from the DLBCL lymph node was labeled with bi-otin and hybridized with expression profile microarray,and the data of expressed genes were obtained.Preliminary bioinformatics analy-sis was performed.Results： Using expression profile microarray,164 genes encoded by chromosome 12 found expressed in DLBCL lymph node.The classification of most detected gene is unclear.Conclusion： The profile of expressed genes encoded by chromosome 12 by using expression profile microarray may provide useful information for DLBCL.
出处
《现代生物医学进展》
CAS
2012年第16期3049-3052,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金(81101571)
