颊粘膜癌与舌癌差异基因表达的对比分析

Differences of Gene Expression Profile between Buccal Mucosa Cancer and Tongue Cancer

目的:研究颊粘膜癌(Buccal Mucosa Cancer,BMC)与舌癌(Tongue Cancer,TC)的基因差异表达,探讨BMC与TC发生发展的基因学基础。方法:应用cDNA芯片技术对5例BMC和5例TC组织mRNA检测,通过芯片杂交、生物信息学处理,找出两者间差异表达基因。结果:BioStarH-40芯片发现差异表达基因503条,差异表达基因占12.9%,其中表达增强274条(显著增强69条),表达降低229条(显著降低54条)。结论:MBC与TC基因表达比较,差异有统计学意义,这些差异可能在两类肿瘤不同的生物学行为中起重要作用。

Objective： To determine the difference in the gene expression between human buccal mucosa cancer（BMC） and tongue cancer（TC）.Methods： cDNA chip was used to detect the mRNA of cancer tissue from 5 BMCs and 5 TCs.After profile bloted and handled by bioinformation,the gene expression of these two kinds of lesions was examined.Results： Using the BioStarH-40 profile,503 different expression of genes were found.The rate of different genes was 12.9%,of which the exprssion of 274 genes increased（69 genes significantly increased） and 229 genes decreased（54 genes significantly decreased） in BMC tissue than that in TC.Conclusions： The gene expression of BMC and TC was different,which may contribute to the different biological behavior of these two kinds of lesions.