脊髓损伤后大鼠行为学改变及早期腓肠肌的变化

Change of Ethology and Gastronomies of Rats after Spinal Cord Injury

目的探索脊髓损伤后大鼠行为学改变及早期腓肠肌变化。方法 108只Wistar大鼠,其中38只为假手术组(n=38),其余制作脊髓切除模型,分为损伤组(n=38)和康复训练组(n=32)。应用BBB法评价大鼠脊髓损伤后不同时间点的行为学变化;通过免疫组织化学法观察腓肠肌的变化。结果康复训练组BBB评分从术后3周开始高于损伤组,但两组得分始终未超过10分。Dystrophin免疫荧光染色显示,脊髓损伤后损伤组和康复训练组腓肠肌肌纤维横截面积均减小,但康复训练组萎缩程度较轻。结论脊髓损伤后大鼠后肢运动功能可出现一定的自发性恢复,康复训练有利于脊髓损伤大鼠运动功能的恢复,并能减缓后肢肌肉萎缩。

Objective To explore the alteration in ethology and change of muscle fibers of gastronomies of rats following spinal cord injury（SCI）.Methods 108 Wistar rats were divided into sham operation group（n=38） and SCI group（n=38） and rehabilitation group（n=32）.The ethology was evaluated by Basso,Beattie,and Bresnahan Locomotor Rating Scale（BBB Scale）,and the alteration of muscle tissue＇s reactivity was observed by immunohistochemistry staining.Results The BBB scores of rehabilitation group were higher than SCI group 3 weeks after injury,but all was lower than 10.The cross section areas of gastrocnemius in both groups were getting smaller,but the rehabilitation group was slighter.Conclusion Spontaneous recovery occurred in some degree for rats＇ hind limb after spinal cord injury.Rehabilitation training is beneficial for the recovery of rats＇ motor function,and alleviates the atrophy of hind limb after spinal cord injury.