干扰素/维甲酸联合应用诱导细胞凋亡相关基因-19在小鼠植入前胚胎中的表达

Expression of Gene Associated with Retinoid-interferon-induced Mortality-19 in Preimplantation Embryo of Mice

目的研究干扰素/维甲酸联合应用诱导细胞凋亡相关基因-19(GRIM-19)在小鼠植入前胚胎中的表达情况,探讨GRIM-19在早期胚胎发育中的动态变化。方法采用Western blot分析和Real-time PCR检测小鼠植入前胚胎(2-细胞期、4-细胞期、8-细胞期、桑葚胚和囊胚期)中GRIM-19蛋白和mRNA水平。结果 GRIM-19在小鼠植入前各期胚胎中均有表达,其蛋白和mRNA水平从2-细胞期逐渐增高,至8-细胞期达高峰,随后呈下降趋势。结论小鼠植入前胚胎中GRIM-19的表达量随发育时程的改变而改变,提示GRIM-19在早期胚胎发育过程中具有一定的作用。

Objective To study the expression of gene associated with retinoid-interferon-induced mortality-19 （GRIM-19） in preimplantation embryo of mice and explore its role in embryonic development. Methods The protein and mRNA expressions of GRIM-19 in 2-cell, 4-cell, 8-cell, morula, and blastocyst phases of mice preimplantation embryo were detected by Western blot analysis and Real-time polymerase chain reaction （PCR）. Results GRIM-19 was continuously expressed in every stage of preimplantation embryo of mice. Western blot analysis and Real-time PCR demonstrated a gradual increase of GRIM-19 expression from 2- cell, which reached a peak in 8-cell phase and then decreased progressively. Conclusions The expression of GRIM-19 in mouse preimplantation embryos changes as at different developmental phases. GRIM-19 may play an important role during embryonic development.