多种梅毒检测方法的比较

Comparison of A Variety of Detection Methods for Syphilis

目的应用多种不同的血清学检测方法测定血清标本梅毒抗体并评价检测结果。方法收集梅毒患者血标本100例,分别采用酶联免疫吸附测定法(ELISA)、梅毒螺旋体明胶颗粒凝集试验(TPPA)、胶体金快速检测试验(ACONSYP)、甲苯胺红不加热血清试验(TRUST)检测血清梅毒。结果先进行TP-ELISA初筛检测为梅毒阳性者的100例患者血清中,其他几种方法都是阳性者94例,其中TRUST阳性94例,TPPA阳性98例,SYP阳性96例。但在120例非梅毒对照组中,经TP-ELISA检测阴性人数为114例,特异性为95%。结论多种方法均存在一定的假阴性和假阳性,对于TP-ELISA阳性标本,为了提高梅毒检出率,要做TRUST和TPPA复检。

Objective Application of a variety of different serological detection methods to determine syphilis antibodies of the serum samples and evaluation of test results.Methods 100 blood specimens collected syphilis patients,respectively,using enzyme-linked immunosorbent assay（ELISA）,treponema pallidum gelatin particle agglutination（TPPA）,colloidal gold rapid testing（ACONSYP）,toluidine red unheated serum test（TRUST） serum syphilis.Results TP-ELISA screening test for syphilis-positive serum of 100 patients,several other methods are positive,94 TRUST positive and 94 of TPPA positive 98,the SYP positive 96.The control group of 120 non-syphilis by the number of TP-ELISA tested negative for 114 and a specificity of 95%.Conclusion There are a variety of methods of false negative and false positive for TP-ELISApositive samples,in order to improve syphilis detection rate,do TRUST and TPPA re-examination.