皱纹盘鲍内脏脂质分析

Analysis of the viscera lipid of Haliotis discus hannai

对皱纹盘鲍内脏脂质进行了分析。采用Folch法提取内脏总脂,硅胶柱层析对中性脂及磷脂进行分离;TLC法对中性脂、磷脂不同组分进行分离与其主要组分的制备;10%硫酸—甲醇对样品进行甲酯化,通过气相色谱法对其进行脂肪酸分析比较。结果表明,皱纹盘鲍内脏中磷脂含量可达31.58%,不饱和脂肪酸在磷脂中含量显著高于中性脂中含量。磷脂中含有一定量的缩醛磷脂,磷酯酰乙醇胺(PE)中缩醛型磷脂含量可达50%。研究表明,皱纹盘鲍内脏中含有丰富的磷脂,多不饱和脂肪酸,并含有一定量的缩醛磷脂,具有较好的食用和药用价值,应得到充分利用。

In this study, we analyzed the lipid of viscera of Haliotis discus hannai. The total lipids of the abalone viscera were extracted using Folch method. Separated neutral lipids and phospholipids by Silica gel column chromatography. Different components of neutral lipids and phospholipids were separated by TLC, and the main components were prepared by TLC. The composition of fatty acids was analyzed by GC, derived with 10% H2SO4-CH3OH solution, then compared the composition of fatty acids of different components. The phospholipid content in Abalone viscera is up to 31.58%, the content of unsaturated fatty acids in the phospholipid is significantly higher than that in the neutral lipid. Phospholipids contain a cer-tain amount of plasmalogen, especially in the phosphatidylethanolamine. The Abalone viscera is rich in phospholipids and unsaturated fatty acids, and it contains a certain amount of plasmalogen. The abalone viscera is the potential material for diet and medicine, which should be fully utilized.