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RT-LAMP方法在血液HCV筛检中的初步应用 被引量：1

Application of Reverse Transcription Loop-Mediated Isothermal Amplification Assay in Blood HCV RNA Screening
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摘要 目的探讨逆转录环介导等温扩增(reverse transcription loop-mediated isothermal amplification,RT-LAMP)方法在血液丙型肝炎病毒(hepatitis C virus,HCV)筛检中的应用价值。方法利用RT-LAMP法和ELISA法分别对450份血液标本进行HCV检测。结果 RT-LAMP方法检出3份HCV RNA阳性,加荧光染料(SYBR Green I)染色后进行结果判定;ELISA法检出2份抗HCV阳性标本;RT-LAMP法相比ELISA法检出率更高。结论 RT-LAMP法有应用于血液筛检HCV RNA的潜力。 Objective To evaluate the application value of reverse transcription loop-mediated isothermal amplification（RT-LAMP） assay in blood hepatitis C virus（HCV） screening.Methods Four hundred and fifty specimens of blood were detected for HCV by RT-LAMP assay and ELISA.Results After RT-LAMP reaction,positive results were observed by SYBR Green I staining in three specimens of blood,while only two positive specimens were detected by ELISA.The detection rate of RT-LAMP assay for HCV RNA is higher than that of ELISA.Conclusions The RT-LAMP assay is a promising approach for blood HCV RNA screening.
作者 王世华 高菊红
机构地区 广州军区广州总医院输血科 广州市越秀区妇幼保健院东山分院
出处 《实用预防医学》 CAS 2012年第6期917-918,共2页 Practical Preventive Medicine
关键词 丙型肝炎 逆转录环介导等温扩增 血液筛检 HCV RT-LAMP Blood screening
分类号 R446.11 [医药卫生—诊断学]
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参考文献10

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共引文献139

同被引文献19

  • 1申振华,陈文明,李爱华,赵建丽.LAMP技术在医学检验中的应用[J].医学检验与临床,2010(4):97-98. 被引量：6
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二级引证文献4

实用预防医学
2012年 第6期

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