摘要
对箭叶淫羊藿中总黄酮进行水提取,以淫羊藿甙为对照,用紫外分光光度法在270nm处测定淫羊藿总黄酮含量,通过正交试验确定淫羊藿总黄酮的最佳提取工艺。结果显示,2%Na2CO3水溶液,15倍量,提取3次,每次1.5h,淫羊藿总黄酮的提取率为97.92%,该工艺简便、可行、生产成本低,可供工业化生产参考。
To optimize the extraction procedure of the total flavonoids from Epimedium sagittatum Maxin, Extraction procedure was screened by orthogonal test according to the eontent of the total flavonoids which was measured through UV speetrophotometry at 270 nm using Icariin as control.The results showed that the optimized extraction procedure was as follows 2% Na2CO3, 15 times volume of the weight of the dried material, extraction 1.5 h for 3 times. The extracting ratio of total flavonoids was 97.92%. The procedure was so simple and feasible that it can be used as reference of production.
出处
《天津农业科学》
CAS
2012年第3期39-41,共3页
Tianjin Agricultural Sciences
关键词
淫羊藿
总黄酮
提取工艺
正交试验
Epimedium sagittatum Maxim total flavonoids
extraction procedure
orthogonal test