分子超光谱成像系统应用于糖网病药物疗效研究

Evaluation of erythropoietin efficacy on diabetic retinopathy based on molecular hyperspectral imaging(MHSI) system

将成像技术和光谱技术相结合,再配合显微镜技术,研制了基于AOTF(acousto-optic tunable filters)的分子超光谱成像系统.使用该系统采集了正常、糖尿病和EPO(erythropoietin,促红细胞生长素)药物治疗的大鼠视网膜组织切片的分子超光谱图像数据.通过对正常组、糖尿病组、药物治疗组共30例样本的分子超光谱图像数据进行处理,获得了3组样本的单波段图像和伪彩色合成图像,并提取了各组样本外核层的典型透射光谱曲线.从图像上分析各组ONL(outer nuclear layer,外核层)的厚度,由大到小依次为正常组、治疗组、糖尿病组,由于糖尿病会引起视网膜外核层细胞凋亡和厚度减少,实验结果表明经EPO治疗后可增加视网膜外核层的厚度.从光谱上分析各组的透射强度,糖尿病大鼠视网膜外核层组织在550～1000 nm光谱范围内的透射强度整体高于正常组,经EPO治疗后,透射强度介于正常组和糖尿病组之间;通过光谱相似性分析,治疗组与正常组之间的光谱相似性高于糖尿病与正常组.实验结果表明EPO能减少视网膜外核层细胞凋亡,对糖网病大鼠有一定的疗效.因而分子超光谱成像系统可以作为一种新的手段,辅助科研人员对糖网病的发病机理和致盲原因及药物疗效进行研究.

Integrate imaging technology, spectrum technology and microscope, a novel molecular hyperspectral imaging （MHSI） system based on AOTF （acousto-optic tunable filters） was presented. The system was used to capture hyperspectral images of rat retinal sections selected from three groups： normal （ N）, diabetic （ D）, and EPO-treated （ E0 ）. By processing hyperspectral images data of 30 samples, single band images and the pseudocolor images of N、 D、 E0 group are gained. Typical spectra curves of the ONL cells of N,D,E0 are extracted. By analyzing these images, descending order of the ONL thickness is N、E0、D. The experimental results cite that the ONL thickness of E0 group will be increased after EPO treated. By analyzing these spectrums, transmission intensity of D group is generally higher than that of N group in the spectral field from 550 nm to 1000nm and transmission intensity of E0 group is in the middle of D and N. By analyzing spectrum relations, results show the spectral signature of the E0 group was more similar to that of N than that of D to N. The above results suggest EPO may prevent cell apoptosis and has protective effect on rat DR at a certain level. MHSI system may probably provide a potential approach to researching pathogenesis 、causes of blindness and drug efficacy of DR in future.