栽培基质对利用三叶草扩繁AM真菌的影响

Effects of Culture Substrates on Propagation of Arbuscular Mycorrhizal Fungi Using Trifolium repens L. as Host

采用4×4双因子设计,研究不同栽培基质和AM真菌菌株对三叶草的生长与营养状况及AM真菌侵染的影响。结果表明:基质种类和AM真菌均显著影响三叶草接种AM真菌的侵染率,纯基质中接种BEG-141和BEG-168菌株三叶草的AM真菌侵染率达到90%以上,与沙基质中AM真菌侵染率无显著差异,但纯基质中三叶草的生物量显著大于沙,说明纯基质可以作为AM真菌扩繁的培养基质,这为AM真菌的规模化生产提供了试验依据。

Different culture substrates and AM fungal strains on the growth and nutrition of Trifolium repens L. as well as infection of AM fungi were investigated by 4×4 two factor design experiments in the present work. The results showed that different substrates and AM fungi highly influenced the mycorrhizal infection rate. Root colonization rate of Trifolium repens L. , inoculated with BEG-14 and BEG-168 and cultured in pure sub- strate without sand, was higher than 90% , which was not significantly different with those cultured in sand. Biomass of the Trifolium repens L. cultured in pure substrate without sand was significantly elevated in com- parison with that in sand. The obtained evidences suggested that the pure substrate adopted in the experi- ments might be optimal culture substrate for AM fungi propagation, thus can provide an experimental base- ment for enlargement production of AM fungal inoculum.