摘要
采用高效液相色谱仪,在指纹图谱条件下测定了20批何首乌药材中二苯乙烯苷的含量。所选色谱柱Ag-ilent SB-C18(5μm,4.6 mm×250 mm),检测波长280 nm,流动相乙腈-0.4%磷酸水溶液梯度洗脱,流速1.0mL/min,柱温25℃,分析时间60 min。经精密度、稳定性、重复性试验考察,RSD均小于3%,平均回收率为100.33%,表明所建立的方法稳定可靠,可满足企业在进行指纹图谱研究的同时进行二苯乙烯苷的含量测定。
Abstract： The stibene glycosides content was determined in 20 samples of Polygonum multiflorum Thunb un- der the conditions of HPLC fingerprint. The method was established as follows： Chromatographic column Agi- lent SB - C18 （5μm, 4.6mm×250mm） ; Detective wavelength 280nm; Mobile phase acetonitrile - 0.4% water solution of phosphori acid graditent elution; velocity 1.0mL/min; Column temperature 25 degree centi- grade ;analysis time 60min. The RSD were all less than 3% in precision, stability and repeatability testing study, and the average recovery rate was 100.33%. It indicated that the established method was stable and reliable, which made it possible for enterprises to determine the content of stilbene glucoside while studying fingerpr/nts.
出处
《山地农业生物学报》
2012年第3期264-267,共4页
Journal of Mountain Agriculture and Biology
基金
国家科技支撑计划[(2009BA174B01-2-12)]
贵州省优秀科技教育人才省长专项资金项目(黔省专合字2010)
贵州省中药材资源保护与可持续利用研究创新团队[黔科合人才团队(2010)4006]
贵州省中药材繁育与种植工程实验室建设[黔发改高技(2009)2805]
贵州省药用植物与重点实验室建设[黔科合计Z字(2010)4015]
关键词
二苯乙烯苷
含量测定
指纹图谱
何首乌
Stilbene glucoside
content determination
fingerprint chromatogram
Polygonum multi-florum Thunb
