γ-Fe_2O_3纳米颗粒对鲤鱼各组织中SOD和GSH-Px酶活性的影响

Effects of γ-Fe_2O_3 Nanoparticles on SOD and GSH-Px Activities in the Various Tissues of Carps

为了研究γ-Fe2O3纳米颗粒对鲤鱼各个组织(脑,鳃,肝,肾,脾)的SOD和GSH-Px酶活性的影响,将大小一致的鲤鱼随机分为5组,分别暴露在0.1、1、10、100mg·L-1的γ-Fe2O3纳米颗粒悬浊液中,同时设有空白对照组,暴露30d后,测定各个组织匀浆中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的含量,从而评价γ-Fe2O3纳米颗粒对鲤鱼各组织的氧化损伤作用。结果表明,在实验浓度下(0～100mg·L-1),各组织对纳米颗粒的相对敏感性不同,与对照组相比,鳃、肝和脾组织中的SOD活力降低;而脑、肝和肾组织的GSH-Px含量降低,该结果表明γ-Fe2O3纳米颗粒对鲤鱼内脏组织的抗氧化系统有一定程度的损害。同时,肝组织中SOD和GSH-Px含量变化与γ-Fe2O3纳米颗粒染毒浓度存在一定的剂量——效应关系。上述结果说明肝脏为γ-Fe2O3纳米颗粒对鲤鱼氧化损伤中的靶器官。

In order to study the effect of γ-Fe2O3 nanoparticles on SOD and GSH-Px activity in the various tissues （brain, gill, liver, kidney, and spleen tissues） of carps, the selected carps were randomly divided into 5 treatment groups, which were exposed to different concentrations of γ-Fe2O3 nanoparticles （0, 0.1, 1, 10, 100 mg·L-1） at room temperature. After 30 days exposure, the activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） were measured as indicators of oxidative damage. The results indicated that the sensitivities to the γ-Fe2O3 nanoparticles were different for the selected tissues. Compared with the control group, the SOD contents decreased much in the gill, liver, and spleen tissues, while the GSH-Px contents reduced in the brain, liver, and kidney tissues, suggesting that γ-Fe2O3 nanoparticles could cause certain degree of damage effects on the anti-oxidant systems of the above three mentioned tissues. Meanwhile, only in the liver tissue, dose-effect relationships were observed between γ-Fe2O3 nanoparticle concentrations and the SOD and GSH-Px activities. The results demonstrated that liver can be con- sidered as the target organ for the oxidative damage study of carps.