HPLC测定补阳还五汤总苷中黄芪甲苷和芍药苷的含量

Determination of Astragaloside Ⅳ and paeoniflorin in total glucosides of Buyang Huanwu decoction by HPLC

目的建立高效液相色谱法测定补阳还五汤总苷中黄芪甲苷和芍药苷的含量。方法色谱柱为Kromasil C18柱（4.6 mm×250 mm,5μm）,黄芪甲苷测定：蒸发光散射检测器,流动相为甲醇∶水=80∶20（v/v）;漂移管温度60℃;气体流量1.5 L/min;输出压力0.5 MPa;柱温30℃。芍药苷测定：流动相为甲醇∶水=23∶77（v/v）;流速为1 mL/min,检测波长：218 nm,柱温：30℃。结果黄芪甲苷在0.57～9.10μg时,Y=1.506 3X＋2.409 6,r=0.999 4;芍药苷在0.48~7.70μg时,Y=598.91X-2.334 6,r=0.999 9;其线性关系良好,平均加样回收率分别为99.62%和101.1%,RSD为2.6%和1.7%（n=6）。结论本法快速、简便、准确、灵敏度高,适用于黄芪甲苷及芍药苷的含量测定,并为其总苷物质制备工艺研究提供质量标准依据。

Objective To establish the HPLC conditions for determination of Astragaloside Ⅳ and paeoniflorin in total glucosides of Buyang Huanwu decoction.Methods The Kromasil C18 column（250 mm×4.6 mm,5μm） was used.Astragaloside Ⅳ detection conditions：Evaporative light scattering detector was used;the mobile phase was methanol-water（80：20）（v/v）;temperature of drift tube was 60 ℃;gas flow rate was 1.5 L/min;pressure was 0.5 Mpa and column temperature was 30 ℃.Paeoniflorin detection conditions：the mobile phase was methanol-water（23：77）（v/v）;the flow rate was 1.0 mL/min;the detection wavelength was 218 nm and column temperature was 30 ℃.Results Astragaloside IV was better in linear correlation between 0.57~9.10 μg,Y=1.5063X ＋2.4096,（r=0.9994）.Paeoniflorin was better in linear correlation between 0.48~7.70 μg,Y=598.91X-2.3346（r=0.9999）.The average recoveries were 99.62% and 101.3% respectively,and RSD were 2.6% and 1.7%（n=6） respectively.Conclusion The method is rapid,simple and accurate and has high sensitivity for determination of Astragaloside Ⅳ and paeoniflorin,which can provide quality standards for the study of total glycosides.