河南郑州市男男同性恋HIV-1感染者gag区基因亚型分析

Subtype and Sequence Analysis of Gag Gene of HIV-1 Among Men Who Have Sex with Men in Zhengzhou,Henan Province

为了解河南郑州市男男同性恋人群HIV-1流行株的亚型分布情况,运用反转录及套式PCR方法从40例已经被确认为HIV-1阳性的MSM的全血样本中扩增gag全长基因并进行序列测定,应用BioEdit软件对序列进行校对编辑,利用MEGA3.1软件构件系统进化树,同时使用美国Los Alamos国家实验室HIV核酸序列库和美国NCBI提供的在线分析工具进行比对分析,确定基因亚型。结果成功获得24条gag基因序列,亚型分析结果显示,共存在B、CRF01-AE和CRF07-BC三种亚型,其中B亚型8例(33.33%),CRF01-AE亚型10例(41.67%),CRF07-BC亚型6例(25%)。小样本量的流行病学调查显示,河南郑州市同性恋人群中主要存在B,CRF01-AE和CRF07-BC三种亚型,CRF01-AE已成为河南郑州市MSM人群中HIV主要流行亚型,河南地区重组型毒株逐渐占优势,流行情况更加复杂。

To investigate the subtype distribution of human immunodeficiency virus-1 （HIV-1） infection among men who have sex with men （MSM） in Zhengzhou, Henan Province, forty blood samples were collected from HIV-1 carriers, who acknowledged to have sex with men. The complete gag gene was amplified by RT-PCR and nested-PCR and sequenced. All sequences were edited by BioEdit and subtyped by genotyping software. Phylogenetic analysis of gag gene were then performed using the MEGA 3.1 software, the gene distances were calculated by Distance program. There were three different HIV-1 subtypes including B, CRF01-AE and CRF07-BC present among twenty four MSMs in Zhengzhou. Genotyping resuits showed that 33.33 % （8/24） were B, 41.67 % （10/24） were CRF01-AE and 25 % （6/24） were CRF07- BC,and subtype CRF01-AE had become the most prevalent HIV-1 subtype in Zhengzhou, Henan prov- ince. In conclusion, recombinant HIV-1 strains are circulating in Henan province and the epidemiology is complicated.