云南省2009～2010年肠道病毒71型的基因特征分析

Genetic Characteristics of EV71 in Yunnan Province from 2009 to 2010

为了解引起云南省2009～2010年手足口病(Hand,Foot and Mouth Disease,HFMD)的肠道病毒71型(En-terovirus 71,EV71)的基因特征,对从病例中分离到的50株EV71,进行VP1编码区逆转录-聚合酶链反应(Reversetranscription-polymerase chain reaction,RT-PCR)扩增和核苷酸序列测定,构建遗传进化树进行基因型别分析。50株分离株VP1编码区全长均为891个核苷酸,编码297个氨基酸,序列比较证实为EV71。进化分析表明,云南省EV71分离株48株属于C4a亚型,2株属于A型。说明云南省2009～2010年流行的EV71和中国大陆其他地区2004年以来的EV71分离株同属C4a亚型,在不同地区、不同疾病状态下分离的EV71在VPl区全长基因序列上无明显差别,但2009年在云南省出现A基因型的传播。

In order to learn about the genetic characteristic of human enterovirus type71 （HEV71）isolated from cases of Hand,Foot and Mouth Disease（HFMD）in Yunnan Province from 2009 to 2010. 50 isolates form HFMD cases were performed entire VP1 coding region amplification by reverse transcription-polymerase chain reaction and sequencing the nucleotide sequences; then the phylogenetic tree was constructed. The complete nucleotide sequences of region VP1 of the 50 strains were all 891nt length coding 297 amino acids. The result of molecular identification of the 50 strains is HEV71. Phylogenetic analysis indicated that 48 EV71 isolates belonged to subgenotype C4a and 2 EVT1 isolates belonged to genotype A. From 2009 to 2010, the pathogen of HFMD cases were EVT1 strains in Yunnan province, which were co-evolved with isolates from other provinces in mainland of China. There was no significant difference found in the whole sequence of VP1 gene of the strains isolated from different regions or under different diseases occurred,but the spread of genetype A appared in Yunnan Province in 2009.