摘要
本研究将PrP106-126多肽和HeLa细胞共孵育4h和8h,采用Hoechst染色分析发现PrP106-126诱导凋亡细胞细胞核出现不同程度的染色质浓集,固缩及碎裂的细胞凋亡征象。Western blotting检测发现PrP106-126诱导细胞中的多聚ADP核糖聚合酶(poly ADP-ribose polymerase,PARP)降解,提示PrP106-126通过caspase3途径引起细胞凋亡现象。PrP106-126诱导的细胞中14-3-3β在不同孵育时间也出现降解,而Real-time PCR检测14-3-3βmRNA未发生变化。本研究证明PrP106-126通过caspase3诱导HeLa细胞凋亡,并可导致抗凋亡蛋白14-3-3β的降解而加速凋亡的形成。
To investigate changes of 14-3-3β from apoptosis induced by PrP106-126 polypeptide, H eLa cell was incubated with PrP106-126 for 4h or 8h. Nucleus changes and the expression of PARP were detected differently by Hoechst staining and Western blotting. Expressing of protein and mRNA from 14-3-3β was determined by Western blotting and Real-time PCR. The results show that typical nucleus pyknosis and chip of apoptosis and degradation of PARP were induced by PrP106-126 peptide in HeLa cells. Degradation of 14-3-3β appeared in apoptosis groups induced by PrP106-126 peptide. However, 14-3-3β mRNA did not display any changes in apoptosis groups. This study indicated that degradation of antiapoptosis protein 14- 3-3β induced by PrP106-126 peptide may be one of pathogenesis mechanism of prion disease.
出处
《病毒学报》
CAS
CSCD
北大核心
2012年第4期414-417,共4页
Chinese Journal of Virology
基金
传染病重大专项(2009ZX10004-101
2008ZX10004-002)
重点实验室发展基金(2008SKLID102
2011SKLID104)资助
