有机镓促进卵巢切除大鼠骨折愈合及生长的实验研究

The experimental research of organic gallium promoting fracture healing and growth in ovariectomized rats

目的本研究的目的是证实有机镓对卵巢切除大鼠骨折愈合的作用。方法 40只雌性Wistar大鼠被分为2组:(1)假手术组(对照n=10只),(2)卵巢切除组(n=30只),无菌条件下腹侧入路行完整双侧卵巢摘除;假手术组摘除与卵巢重量相同的卵巢周围脂肪组织各1块。12w后对40只大鼠制造开放性骨折并予克氏针内固定。术后分为假手术组(n=10)和一个卵巢切除组(n=15)用PBS治疗,另外一个卵巢切除组(n=15)用有机镓治疗。治疗共持续4w,取骨折愈合的大鼠股骨分别进行micro-CT测定骨小梁组织结构;组织形态学测定骨组织愈合面积;生物力学测定愈合股骨的最大负荷载力;骨矿含量检测评价钙盐含量。结果经过4w治疗,micro-CT显示有机镓治疗组平均骨小梁厚度(Tb.Th)、平均骨小梁数目(Tb.N)均明显高于对照组(P<0.05)。卵巢切除组的愈合组织骨面积较假手术组降低9.2%,有机镓治疗组比卵巢切除组高34.9%(P<0.05)。有机镓治疗组股骨骨折愈合强度显著高于卵巢切除组,最大负荷载力增加50.6%,结构强度增加36.5%,能量吸收增加90.9%,但是均低于假手术组。与卵巢切除组相比,有机镓治疗组显著提高愈合组织的骨矿含量。结论有机镓能够抑制骨折后的骨量丢失,促进骨折愈合组织的生长,改善骨小梁三维结构及骨组织的力学性能,可用来促进骨质疏松性骨折的愈合并改善骨质量,预防再骨折。

Objective To verify the effect of organic gallium on the fracture healing in ovariectomized rats. Methods Forty Wistar female rats were divided into 2 groups： sham-operated group （SHAM, n = 10） and ovariectomized group （OVX, n = 30）. Bilateral ovary resection was performed under sterile condition. Equal weight of the peri-ovary fat tissue was cut in rats of SHAM group. Fractures were produced by open surgery in 40 rats and the fractures were internally fixed. After the surgery, rats were divided into Sham （n = 10） and OVX group which were treated with PBS. And the other OVX group （n = 15 ） that were treated with organic gallium （OG）. The treatment lasted for 4 weeks. The healed femurs were assessed for skeletal structure with micro-CT, healing area with histomorphometry, maximal loading with biomechanical testing, and the content of mineral substances in bones. Results Micro-CT showed that the treatment with OG increased BV/TV and trabecular thickness, and decreased trabecular separation in OVX animals. Histomorphometry evaluation demonstrated that OG increased callus amount and strength. OG also improved the biomechanical properties of bone healing. Quantitative analysis of the bones from animals in OG group revealed significantly increase of mineral contents compared to bones from OVX groups. Conclusion This study suggests that OG may represent a potential alternative medicine in promoting fracture healing in ovariectomized rats.