胰岛因子1在维吾尔族妇女子宫颈癌中的表达及相关研究

The expression and related research of ISL1 in cervical cancer of the Uyghur women

目的探讨胰岛因子1(ISL1)在维吾尔族妇女宫颈病变组织中的表达情况及其临床意义。方法采用半定量RT-PCR技术检测ISL1在维吾尔族妇女宫颈鳞癌(CSCC,15例)、宫颈上皮内瘤变(CINⅡ～Ⅲ,13例)和正常宫颈组织(12例)中的mRNA表达情况;采用免疫组织化学(S-P)法检测维吾尔族妇女CSCC(42例)、CINⅢ(23例)和正常宫颈组织(20例)中ISL1蛋白的表达情况。结果 CSCC组织中ISL1的mRNA表达水平(0.153 0±0.122 3)明显低于正常宫颈组织(0.271 3±0.763 3),差异有统计学意义(P<0.05),CINⅡ～Ⅲ组织中ISL1的mRNA转录表达水平(0.225 3±0.132 5)与CSCC及正常宫颈组织比较,差异均无统计学意义(P>0.05)。免疫组织化学分析结果显示,CSCC组织中的ISL1蛋白表达水平(16.67%)显著低于正常宫颈组织(55.00%),差异有统计学意义(P<0.01),CINⅢ组织中ISL1的表达水平(26.09%)与CSCC及正常宫颈组织比较,差异均无统计学意义(P>0.05)。结论 ISL1蛋白和mRNA在维吾尔族妇女宫颈癌中呈低表达,对该基因进行检测有利于宫颈癌的早期诊断、早期筛查。

Objective To investigate the expression level of ISL1 in normal cervix,cervical intraepithelial neoplasia and cervical cancer and their clinical signficances of Uyghur women.Methods Semi-quantitative reverse transcription PCR（RT-PCR） was used to examine the mRNA expression level of ISL1 in 15 CSCC,13 CIN Ⅱ~Ⅲ and 12 normal control tissue samples of Uyghur women.The protein expression of 42 cervical cancer,23 CINⅢ and 20 normal cervix tissue samples of Uyghur women were examined.Results mRNA expression level of ISL1 in CSCC tissue（0.153 0±0.122 3） was significantly lower than in normal cervix tissue（0.271 3±0.763 3）,with statistical significance（P0.05）.Immunohistochemistry showed that the protein expression level of ISL1 in CSCC（16.67%） was significantly lower than in cervicitis（55.00%,P0.05）.Conclusion ISL1 is underexpressed in cervical cancer,which may help to early diagnosis and screening of cervical cancer.