水通道蛋白1、3在非肌层浸润性膀胱癌组织中的表达及其与复发现象的相关性

Expression and correlation with recurrence of 1,3 aquaporins in non-muscle invasive bladder cancer

目的研究水通道蛋白1、3(AQP1、AQP3)在非肌层浸润性膀胱癌组织中的表达及其与膀胱癌复发现象的相关性。方法采用免疫组织化学S-P法、Wester blot蛋白免疫印迹法检测15例经尿道膀胱癌电切术后非肌层浸润性膀胱癌组织及15例复发组织水通道蛋白1、3的表达量,分析两者在复发前及复发前后表达量的差异与复发时间的相关性。结果复发后水通道蛋白1、3的表达量较复发前有所增加,且复发前蛋白表达量高的容易复发,其蛋白表达增加的量与随访的复发时间之间呈负相关。结论水通道蛋白1、3的表达量在复发后的明显增加,其增加的量及初发后蛋白表达量与随访的复发时间相关,AQP1、AQP3可成为检测膀胱癌早期复发的有效指标之一。

Objective To study the expression and correlation with recurrence of 1,3 aquaporins in non-muscle invasive bladder cancer.Methods The expression of 1,3 aquaporins in non-muscle invasive bladder cancer tissue of 15 patients after transurethral resection of urinary bladder cancer was detected using immuno-histochemistry S-P method,Western blot and analyzed the correlation between before and after the relapse.Results The expression of aquaporin 1 and 3 increased in after relapse compared with the amount of the increase before relapse,and recurrence of high levels of protein expression before,easy to relapse,and there is a negative correlation between amount of increasing protein expression and the recurrence time of following-up.Conclusion The amount of the expression of aquaporin 1 and 3 apparently increased in recurrence,the increasing amount and primary protein expression have negative correlation with the recurrence time of following-up,AQP1,AQP3 can be one of the effective indicators which is used to detect the early recurrence of bladder cancer.