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HIF-1α、MT、Slug和E-cadherin在人乳腺癌中的表达及其意义 被引量:14

The expressions and significances of HIF-1α,MT,Slug and E-cadherin in human breast cancer
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摘要 目的:探讨HIF-1α、MT、Slug和E-cadherin在人乳腺癌中的表达及其意义。方法:采用免疫组化SP法,检测术前未使用放疗、化疗及激素治疗的69例乳腺癌组织中HIF-1α、MT、Slug和E-cadherin的表达情况。采用RT-PCR方法检测乳腺癌细胞系MCF-7和MDA-MB-231中HIF-1α、MT、Slug和E-cadherin mRNA的表达情况。结果:免疫组化结果表明:在69例乳腺癌组织中,HIF-1α、MT、Slug和E-cadherin的阳性率分别为71.01%、42.03%、43.48%和50.72%。HIF-1α、Slug和E-cad-herin的表达对于TNM分期和腋淋巴结转移有显著性差异(P<0.05);MT的表达对于TNM分期有显著性差异(P<0.05)。在69例乳腺癌组织标本中HIF-1α与MT表达呈显著正相关(r=0.285,P<0.05),MT与Slug呈显著正相关(r=0.379,P<0.01),Slug与E-cadherin呈显著负相关(r=-0.422,P<0.01)。RT-PCR半定量结果显示:HIF-1α、MT、Slug和E-cadherinmRNA在人乳腺癌细胞系MCF-7和MDA-MB-231中均有表达,但在恶性程度更高的MDA-MB-231细胞中HIF-1α、MT、Slug表达较高,而E-cadherin表达较低,差异具有显著性(P<0.05)。结论:HIF-1α、MT、Slug和E-cadherin在乳腺癌中的表达情况可作为监测乳腺癌发展的指标。 Objective:To investigate the expressions and significances of HIF- 1α, MT, Slug and E-cadherin in human breast cancer. Methods: Immunohistochemistry was used to analyze the expression of HIF-1α, MT, Slug and E-cadherin in 69 tissue samples of breast cancer patients without any radiotherapy, chemotherapy and hormone therapy. The mRNA expressions of HIF-1 α, MT, Slug and E-cadherin in breast cancer cell lines MCF-7 and MDA-MB-231 were detected by RT-PCR analysis. Results:In 69 tissue samples of breast cancer, the positive expression rates of HIF-1α, MT, Slug and E-cadherin were 71.01%, 42.03% , 43.48% and 50.72%. The expressions of HIF-1α, Slug and E-cadherin have significant correlations with TNM stage and axillary lymph node metastasis (P 〈 0.05 ), the expression of MT has a significant correlation with TNM stage ( P 〈 0.05 ). In 69 tissue samples of breast cancer, the expression of HIF-1 ct has a positive correlation with that of MT ( r = 0. 285, P 〈 0.05 ) , the expressions of MT and Slug were significantly positively correlated (r = 0. 379, P 〈 0.01 ), and the expression of Slug was significantly negatively correlated with that of E-cadherin (r = -0.422 ,P 〈 0. 01 ) in breast cancer. RT-PCR analysis showed that HIF-1α, MT, Slug and E-cadherin mRNA were expressed in breast cancer cell lines MCF-7 and MDA-MB-231. The mRNA expression levels of HIF-1α, MT and Slug was higher and the expression levels of E-cadherin was lower in MDA-MB-231 cell line than in MCF-7 cell line ( P 〈 0.05 ). Conclusion : The expressions of HIF-1α, MT, Slug and E-cadherin might be used as markers for development of breast cancer.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2012年第6期502-506,共5页 Chinese Journal of Immunology
基金 国家自然科学基金面上项目(81072183) 重庆市自然科学基金重点项目(CSTC 2011BA5038) 重庆医科大学重点科研课题(XBZD201002)资助
关键词 乳腺癌 HIF-1Α MT SLUG E-CADHERIN Breast cancer HIF-1α MT Slug E-cadherin
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参考文献15

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