登革病毒感染对T细胞和NK细胞表达细胞因子的影响

Effects of Dengue Virus Infection on Expression of Cytokines in T cells and NK Cells

【目的】本文探讨登革病毒体外感染人外周血单个核细胞后对T细胞和NK细胞表达细胞因子的影响,并初步探讨登革病毒感染引起的免疫效应。【方法】用登革2型病毒(DEN-2)感染健康人外周血单个核细胞(PBMC),流式细胞术检测登革2型病毒后T细胞和NK细胞的表型及细胞因子的表达水平。【结果】DEN-2感染18 h后,T细胞明显表达CD69、IFN-γ、TNF-α和perforin,NK细胞表达CD69、IFN-γ和perforin;随着感染复数(MOI)增加,表达perforin和IFN-γ的T细胞数及表达perforin的NK细胞数上升;随着感染时间的延长,表达perforin和IL-4的T细胞数及表达perforin的NK细胞数逐渐上升,而表达IFN-γ的T细胞数及表达IFN-γ的NK细胞数逐渐下降;表达perforin的T细胞亚型主要为CD8+T细胞。【结论】发现登革病毒在体外能够诱导T细胞表达CD69、IFN-γ、TNF-α、perforin和IL-4,及NK细胞表达CD69、IFN-γ和perforin,提示T细胞、NK细胞可能在抗登革病毒感染或免疫损伤中起着重要的作用。

[Objective] To investigate the expression of cytokines in T cells and NK cells from human peripheral blood mononuclear cells in response to dengue virus in vitro, and to explore the immune response after dengue virus infection. [ Methods ] Flow cytometry was used to detect the phenotype and cytokines of T cells and NK cells from PBMC after DEN-2 infection. [Results] T cells express CD69, IFN-γ, TNF-α, perforin, and NK cells express CD69, IFN-γ, perforin 18 hours after DEN-2 infection. The numbers of IFN-γ and perforin expressing T ceils and perforin expressing NK cells have a positive correlation with dengue virus dose. The numbers of perforin and IL-4 expressing T cells and perforin expressing NK cells increase, whereas the numbers of IFN-γ expressing T ceils and NK cells decrease follow infection times. T cells expressing perforin are mainly CD8^＋T ceils. [Conclusion] With in vitro infection by dengue virus, T cells can express CD69, IFN-γ, TNF-α, perforin and IL-4. And NK cells can express IFN-γ and perforin at the same time. We can conclude that T and NK cells may play an important role in the anti-dengue virus infection or immunopathology.