近红外光谱技术用于川红活血胶囊提取过程的研究

Studies on NIR Spectroscopy of Chuanhong Huoxue Capsules Extraction Process

目的用声光可调滤光器(AOTF)-近红外(NIR)光谱法在线分析中药新药川红活血胶囊提取过程中芍药苷的含量。方法在线收集川红活血胶囊提取样品,建立其芍药苷含量数据库,同时采集近红外光谱图谱,用偏最小二乘法(PLS1)分别建立近红外光谱与芍药苷含量数据之间的校正模型,并对在线过程中收集的预测集样品进行含量预测来验证所建模型。结果提取液近红外光谱与含量数据之间的校正模型相关系系数R2为0.992,外部样品预测模型相关系系数R2为0.995,外部样品预测相对偏差为4.25%;该方法用于芍药苷含量测定的精密度RSD为2.70%,稳定性RSD为2.07%,预测回收率为96.7%。结论 AOTF-NIR光谱技术用于川红活血胶囊提取液芍药苷含量分析快速、直接,并能实现现场分析。

Objective To establish a method for on-site analysis of paeoniflorin content of Chuanhong Huoxue Capsules Extracting process with acousto optictunable filter（AOTF）-near infrared （NIR） spectroscopy technology. Methods To collect fluid samples on- site and to establish database of paeoniflorin content and NIR spectroscopy; then calibration model of NIR spectroscopy- paeoniflorin content was established with partial least squares （PLS1）;to verify the calibration model prediction with samples collected online. Results Correlation coefficients R2 between calibration model and inside data were 0. 992 and 0. 995; average relative deviations of prediction and outside sample were 4. 25%;method precision RSD were 2.70%; sample stabilities in 5 h were 2. 07%;forecast recoveries were 96. 7%. Conclusion NIR spectroscopy for on- site analysis of paeoniflorin content of Chuanhong Huoxue Capsules Extracting process is fast,direct,and to achieve on- site analysis.