病毒性脑炎、脑膜炎多病原的探讨

Study on etiological constitution in viral encephalitis and meningitis in children

为探讨北方地区病毒性脑炎及脑膜炎的病原学构成,采用微量细胞培养法,应用人胚肺二倍体细胞(HEF)、人表皮样癌细胞(Hep-2)、猴肾传代细胞(Vero)及狗肾传代细胞(MDCK)等4种细胞系对86例脑炎、脑膜炎患儿的脑脊液、粪便及咽拭标本进行了病毒分离。结果36例分离到了病毒,阳性率为41.86％(36/86例),其中肠道病毒18例,腺病毒3型(AdV_3)8例,单纯疱疹病毒1型(HSV_1)7例,阳性率分别为20.93％、9.30％、8.14％。此外,还分离到流感病毒(Flu)2例,巨细胞病毒(CMV)1例。证实小儿病毒性脑炎及脑膜炎病原以肠道病毒为主,腺病毒及单纯疱疹病毒次之。

To clarify the possible constitution of multiple pathogens in children with viral encephalitis and meningitis, viruses in the materials from CSF, faeces and throat swabs were isolated in 86 children with viral encephalitis and meningitis by using the way of micro-plate technique via four different susceptible cell lines of HEF, Hep-2, Vero and MDCK. The results showed that the positive culture appeared in 36 of 86 cases (41. 86%). Among them, enteroviruses could be discovered in 18 cases (20. 93%), adenovirus type 3 (AdV3) in 8 cases (9. 3%) and herpes simplex virus type 1 (HSV1) in 7 cases (8.14%). In addition, influ virus was seen in 2 cases and cytomegalovirus in 1 case. It is concluded that in etiological constitution of viral pathogens in children with viral encephalitis and meningitis, enterovirus might play an important role, and subsequently followed by AdV3 and HSV1.