雷贝拉唑对胃黏膜损伤保护的作用

The Protective Effect of Rabeprazole on the Gastric Mucosa in Rats

目的探讨雷贝拉唑对大鼠胃黏膜保护作用。方法在乙醇诱导大鼠胃黏膜损伤前，预先给予雷贝拉唑（20mg／kg）灌胃，L-硝基-精氨酸甲酯（L-NAME，4mg／kg）静脉注射。采用激光多普勒血流计（LDF）测定胃黏膜血流量（GMBF），采用镉粒还原和比色法测定胃黏膜和血浆NO2-／NO3-含量，并观察胃黏膜损伤指数（Ulcerindex。UI）、溃疡坏死组织和中性粒细胞浸润严重程度的变化。结果与模型损伤组比。雷贝拉唑组大鼠UI明显降低（Pd0．01），溃疡坏死组织和中性粒细胞浸润程度明显减轻（P〈0．05）。预先用L-NAME处理后，雷贝拉唑保护胃黏膜损伤作用明显减弱。向胃内灌注雷贝拉唑．可增加GMBF、胃黏膜和血浆NO2-／NO3-，L-NAME可逆转这种作用．但对雷贝拉唑抑制酸分泌作用无明显影响。结论雷贝拉唑对大鼠胃黏膜具有重要的保护作用，一氧化氮（Nitricoxide．N0）介导了这种作用。

Objective To observe the protective effect of rabeprazole on the gastric mueosa in rats. Methods Gastric chamber perfusion with rabeprazole（ 20 mg/kgJand intravenous injections with NoJ-nitro-L-arginine methyl ester（ L- NAME,4 mg/kgJwere administered in rats with gastric mucosal lesion induced by pure alcohol. Gastric mucosal blood flow（GMBF）was assessed with laser Doppler flowmetry（LDF）,gastric mucosal and serum NOz-/NO3- ratio were measured by cadmium granulation reduction and colorimetric method, and the changes of ulcer index and the se- verity of tissues necrosis and neutrophils infiltration were observed. Results The ulcer index of rats with rabeprazole treament was much lower than that of the control group（P^0.01 } with lesser degree of tissues necrosis and neutro- phils infiltration（ P^0. 05 ）. The protective effect of rabeprazole was significantly inhibited by prior administration of L-NAME that. Gastric rabeprazole administration obviously increased GMBF,gastrie mucosal and serum NO2-/ NO3- ratio,and such effect,apart from its action against secretion, could he counteracted by pretreatment with L- NAME. Conclusion Rabeprazole play an important protective role in the gastric mueosa in rats, which nitric oxide （NO）Jis involved in.