重症手足口病合并病毒性脑膜脑炎T淋巴细胞亚群的变化及其临床意义

Change and clinical significance of lymphocyte subsets in children suffered severe hand-foot-mouth disease combined with viral encephalomeningitis

目的探讨淋巴细胞亚群在重症手足口病合并病毒性脑膜脑炎患儿中的变化及其临床意义。方法应用流式细胞仪荧光染色技术检测26例重症手足口病合并病毒性脑膜脑炎和40例无合并症轻型手足口病患儿T淋巴细胞亚群，以30例健康体检儿童做对照。结果重症手足口病合并脑膜脑炎患儿CD3＋和CD4＋淋巴细胞比例以及CD4＋／CD8＋比值均明显低于健康对照组，CD4＋CD25＋淋巴细胞比例显著高于健康组，差异有统计学意义（P〈0．01或P〈O．05），而CD8＋淋巴细胞比例差异无统计学意义（P〉0．05）；无合并症轻型手足口病患儿CD3＋和CD4＋淋巴细胞比例以及CD4＋／CD8＋比值均明显高于健康对照组，CD4＋CD25＋淋巴细胞比例明显低于健康组，差异有统计学意义（P〈O．01），而CD8＋淋巴细胞比例差异无统计学意义（P〉0．05）。结论重症手足口病合并脑膜脑炎患儿免疫功能受损下降，处于免疫抑制状态。

Objective To investigate the change and clinical significance of lymphocyte subsets in children suffered severe hand-foot-mouth disease combined with viral encephalomeningitis. Methods Flow Cytometry and Fluorescence Staining were adopted to detect T lymphocyte subsets in 26 children suffered severe hand-foot-mouth disease com- bined with viral encephalomeningitis, 40 children with mild hand-foot-mouth disease, and 30 cases of health children. Results CD3＋/CD4＋ T cell ratio and CD4＋/CD8＋ T cell ratio of children suffered severe hand-foot-mouth disease combined with viral encephalomeningitis were lower significantly than the health children group, CD4＋/CD25＋ T cell ratio was higher significantly than the health group,CD8＋ T cell ratio difference was no statistical significance. CD3＋/CD4＋ T cell ratio and CD4＋/CD8＋ T cell ratio in mild hand-foot-mouth disease were higher significantly than the health group,CD4＋/CD25＋ T cell ratio was lower significantly than the health group, CD8＋ T cell ratio difference was no statistical significance. Conclusion Children suffered severe hand-foot-mouth disease combined with viral encephalomeningitis was under immunosuppressive condition.