抗心磷脂抗体阳性习惯性流产患者人类白细胞抗原-DQ区域基因多态性的研究

Study on Human Leucocyte Antigen-DQ Region Gene Polymorphism in Cases of Habitual Abortion with Anticardiolipin Antibody

目的 探讨抗心磷脂抗体 (ACL)阳性习惯性流产与人类白细胞抗原 DQ区域 (HLA DQ)等位基因多态性的关系。方法 采用聚合酶链反应 限制性片段长度多态性方法 ,对 30例ACL(+)习惯性流产患者进行HLA DQA1和HLA DQB1基因型的分析 ,并以 90例无不良妊娠史妇女为对照。结果 在DQB1等位基因表达频率中 ,DQB1 0 30 3等位基因表达频率在ACL(+)习惯性流产患者为33.3% ,比对照者明显增高 (P <0 .0 5 ) ;在其他DQB1等位基因表达频率上 ,以及DQA1各等位基因和DQA1 DQB1各单元型表达频率上 ,两者间差异均无显著性 (P >0 .0 5 )。结论 DQB1 0 30 3等位基因与ACL(+)习惯性流产相关 ,可能是ACL(+)习惯性流产的易感基因。

Objective To investigate the relationship between human leucocyte antigen(HLA) DQ region gene polymorphism and habitual abortion with anticardiolipin antibody(ACL). Methods Polymerase chain reaction restrictive fragment length polymorphism(PCR RFLP) was used to type HLA DQA1 and HLA DQB1 alleles in 30 cases of habitual abortion with anticardiolipin antibody and 90 women with normal pregnancy history. Results The frequency of DQB1*0303 was found to be significantly higher in ACL(+) habitual abortion group(33.3%) than those in normal control group ( P <0.05). Whereas, there was no significant difference found not only in frequency of other DQB1 alleles, but also in frequency of DQA1 alleles and DQA1 DQB1 haplotypes between two groups. Conclusion The results show an association between ACL(+) habitual abortion and HLA DQB1*0303, which suggest DQB1*0303 may be susceptible gene to ACL(+) habitual abortion.