膜联蛋白A1在兔骨髓间充质干细胞体外诱导成骨和成脂早期的表达

Expression of annexin A1 in rabbit bone marrow mesenchymal stem cells during their early differentiation into osteoblasts and adipocytes in vitro

背景:骨髓间充质干细胞分化过程中膜联蛋白A1表达变化的研究报道各有不同看法。目的:分析兔骨髓间充质干细胞在体外诱导成骨和成脂过程中膜联蛋白A1基因的表达变化。方法:应用全骨髓贴壁法分离培养骨髓间充质干细胞,分别加入含成骨诱导剂、成脂诱导剂及不添加任何诱导剂的培养基进行培养。结果与结论:膜联蛋白A1基因在成骨诱导过程中与未诱导细胞相比有明显下调趋势,差异有显著性意义(P<0.01),而在成脂诱导剂中则为上升(P<0.01)。成骨诱导剂对细胞生长存在抑制作用并增加细胞凋亡(P<0.01),而成脂诱导剂对细胞生长与凋亡作用较小(P>0.05)。因此排除了诱导剂对细胞的作用之后,推测膜联蛋白A1基因可能与骨髓间充质干细胞体外向脂肪细胞分化存在一定关系,但尚不能肯定其在成骨分化中的作用。

BACKGROUND： Studies have reported different views on the expression of annexin A1 （ANX A1 ） in bone marrow mesenchymal stem cells （BMSCs） during their differentiation. OBJECTIVE： To investigate the expression of ANX A1 in rabbit BMSCs during their differentiation into osteoblasts and adipocytes in vitro. METHODS： BMSCs were isolated by whole bone marrow adherence method, and then the cells were cultured in media with osteogenic inducer, adipogenic inducer and non-inducer respectively. RESULTS AND CONCLUSION： The expression of ANX A1 mRNA was obviously decreased during the cell differentiation into osteoblasts compared with those without differentiation （P 〈 0.01 ）. In contrast, the expression of ANX A1 mRNA was obviously increased in BMSCs cultured in medium with adipogenic inducer （P 〈 0.01 ）. Osteogenic inducer could inhibit the growth of BMSCs to achicve an increase in apoptosis （P 〈 0.01 ）, while the adipogenic inducer had little effects on cell growth and apoptosis （P 〉 0.05）. ANX A1 may play an important role in regulation of BMSCs differentiation into adipocytes in vitro without the role of inducer. However, the association with osteogenic differentiation is uncertain.